Targeting STAT3 alleviates peritoneal fibrosis by regulating glycolysis and mesothelial-mesenchymal transition / 安徽医科大学学报

Objective @#To study the effect and mechanism of high glucose on mesothelial-mesenchymal transition (MMT) of peritoneal mesothelial cells (HMrSV5) , and the protective effect of pharmacological blocking of signal transducer and activator of transcription 3 (STAT3) on rat peritoneal fibrosis (PF) model . @*Methods @#The animals were divided into three groups : the sham group , the model group , and the STAT3 inhibitor group . A miniature per- itoneal dialysis catheter was implanted under the dorsal skin of rat and the rat peritoneal fibrosis model was induced by daily injection of high glucose dialysate . After 10 weeks , HE staining was used to evaluate the histology of the peritoneum , and the level of transforming growth factor-β1 (TGF-β1) in the peritoneum was measured by immuno- histochemistry . HMrSV5 was cultured in high glucose and the optimal stimulation concentration of high glucose was determined by Western blot. High glucose was used to stimulate HMrSV5 after successful transfection with si - STAT3 and Western blot was used to measure the protein level of STAT3 , p-STAT3 , and the key enzymes of glycol- ysis 6-phosphofructo-2-kinase/fructose-2 , 6-biphosphatase 3 (PFKFB3) and lactate dehydrogenase A (LDHA) .@*Results @#HE staining showed that administration of STAT3 inhibitor ( BP-1-102) could inhibit the thickening of subperitoneal tissue and the proliferation of vessels in HG dialysis rats . The expression of TGF-β1 in the rats perito- neum of the model group was significantly higher than that in the sham group , and the level of TGF-β1 was marked- ly lower in the STAT3 inhibitor group compared to the model group (P < 0. 05) . Compared to the control group , high glucose induced the up-regulation of α-smooth muscle actin ( α-SMA) , the down-regulation of E-cadherin and STAT3 activation in HMrSV5 (P < 0. 05) . Mesothelial cells treated with high glucose also exhibited high expres- sion of the key enzymes of glycolysis ( PFKFB3 , LDHA) ( P < 0. 05) , and si-STAT3 can effectively inhibit the overexpression of PFKFB3 and LDHA induced by high glucose ( P < 0. 05) . @*Conclusion @#STAT3 is involved in high glucose-induced HMrSV5 hyperglycolysis and MMT , and targeting STAT3 alleviates peritoneal fibrosis and an- giogenesis during peritoneal dialysis treatment in rats .

Application and development of alveoloplasty in implant-supported full-arch fixed prosthesis / 中华口腔医学杂志

Implant-supported full-arch fixed prosthesis is hot in edentulous therapy currently. Appropriate contour of bone is the premise of good restoration outcome. Alveoloplasty is an important part during treatment procedure. Alveoloplasty can be used to obtain bone platform for implant insertion, create adequate prosthetic space, achieve good Aesthetic effect, and form appropriate soft tissue morphology. The design of alveoloplasty has evolved from traditional plaster models and cone beam CT to three-dimensional (3D) virtual patients. The surgical techniques of alveoloplasty have also undergone the evolution from free-hand to static guide or dynamic navigation. This article elaborates on the concept, purpose and significance of alveloplasty in implant supported full-arch fixed restoration, technology evolution and process to provide reference for clinical practice.

Plasma level and activity of endotoxin in patients with ischemic stroke / 心血管康复医学杂志

Objective:To explore plasma level and activity of endotoxin (LPS)in patients with ischemic stroke (IS). Methods:A total of 80 IS inpatients were regarded as stroke group,and another 50 healthy subjects were treated as healthy control group.Plasma levels of LPS,LPS binding protein (LBP)and sCD14 were measured and compared between healthy control group and IS group on 1d and 3d after stroke.Results:Compared with healthy control group,there were significant rise in plasma levels of LPS,LBP and sCD14 in stroke group on 1d and 3d,P <0.01 all;compared with 1d after stroke,there were significant rise in plasma levels of LPS [(0.29 ±0.07)U/ml vs. (0.38±0.05)U/ml],LBP [(22.13±2.75)μg/ml vs.(27.16±3.75)μg/ml]and sCD14 [(1251.11±179.79)ng/ml vs.(1472.29±158.18)ng/ml]in stroke group on 3d after stroke,P =0.001 all.Conclusion:Plasma level and activity of endotoxin significantly rise in patients with ischemic stroke.It helps clinical physicians to diagnose ische-mic stroke by measuring plasma level and activity of endotoxin.

Plasma level and activity of endotoxin in patients with ischemic stroke / 心血管康复医学杂志

Objective:To explore plasma level and activity of endotoxin (LPS)in patients with ischemic stroke (IS). Methods:A total of 80 IS inpatients were regarded as stroke group,and another 50 healthy subjects were treated as healthy control group.Plasma levels of LPS,LPS binding protein (LBP)and sCD14 were measured and compared between healthy control group and IS group on 1d and 3d after stroke.Results:Compared with healthy control group,there were significant rise in plasma levels of LPS,LBP and sCD14 in stroke group on 1d and 3d,P <0.01 all;compared with 1d after stroke,there were significant rise in plasma levels of LPS [(0.29 ±0.07)U/ml vs. (0.38±0.05)U/ml],LBP [(22.13±2.75)μg/ml vs.(27.16±3.75)μg/ml]and sCD14 [(1251.11±179.79)ng/ml vs.(1472.29±158.18)ng/ml]in stroke group on 3d after stroke,P =0.001 all.Conclusion:Plasma level and activity of endotoxin significantly rise in patients with ischemic stroke.It helps clinical physicians to diagnose ische-mic stroke by measuring plasma level and activity of endotoxin.

Role of SphK1 in renal tubulointerstitial fibrosis and its mechanism / 中国药理学通报

Aim To investigate the effect of sphingo-sine kinase 1 (SphK1 )on unilateral ureteral obstruc-tion(UUO)-induced tubulointerstitial fibrosis and ex-plore the possible mechanism.Methods The CD-1 mice were randomly divided into four groups:sham-op-eration group(Sham),PF-543 treatment control group (Sham +PF-543),model group(UUO)and PF-543 treatment group(UUO +PF-543).On 1 ,3,7 and 1 4 d after operation,eight mice were selected randomly from each group and sacrificed.The protein expressions of SphK1 ,mature TGF-β1 ,FN,ColⅠ,LC3,Beclin1 ,Atg5 and Atg1 2 were observed by Western blot.The histo-logical changes were examined by Masson′s trichrome stain.Immunhistochemistry was performed to measure the levels of expression of SphK1 ,FN and Col Ⅰ. Transmission electron microscope was used to observe the autophagic body.Results SphK1 expression and autophagy were both upregulated in a mouse model of kidney fibrosis induced by UUO. Meanwhile, in-creased mature TGF-β1 and deposition of extracellular matrix(ECM)were observed in tubulointerstitial areas compared with sham-operated mice.After intraperito-neal injection with the SphK1 specific inhibitor PF-543 in UUO mice,enhanced expression of SphK1 and acti-vated autophagy were significantly abrogated.Howev-er,aggravation of renal fibrosis was detected when SphK1 inhibitor PF-543 was applied to suppress SphK1 expression in UUO mice.Conclusion SphK1 activa-tion is renoprotective through the induction of autoph-agy in the pathogenesis of kidney fibrosis.

Bushen Qiangji Granule () medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro / 中国结合医学杂志

<p><b>OBJECTIVE</b>To explore the mechanism of Bushen Qiangji Granule (, BSQJ) in restraining the osteogenic differentiation of ankylosing spondylitis (AS) fifibroblasts.</p><p><b>METHODS</b>Hip joint capsules were obtained from AS patients (n=10) receiving total hip replacement and healthy hip joint capsules from patients with hip fracture (n=10) receiving surgery as a control. Finite fifibroblast lines were established from these tissue samples to observe the effect of BSQJ on suppressing osteogenic differentiation of fifibroblasts. The expression of osteogenic marker gene corebinding factor a1 (Cbfa1) and Smad family proteins were examined by Western blot and real-time quantitative polymerase chain reaction (qPCR).</p><p><b>RESULTS</b>The mRNA expression level of Cbfa1 was significantly higher in AS fibroblasts than that in normal fibroblasts and the expression of pSmad1, pSmad5, Smad4 and Cbfa1 in AS fibroblasts was also higher, demonstrating the activation of the BMP/Smads signal pathway in AS fifibroblasts. BSQJ-medicated serum not only restrained the mRNA and protein expression levels of Cbfa1 and inhibited protein expression level of Smad4 but also decreased the expression quantities of pSmad1 and pSmad5.</p><p><b>CONCLUSIONS</b>BSQJ can inhibit osteogenic differentiation of AS fifibroblasts in vitro by suppressing the activation of the BMP/Smads signal pathway. This may be the important molecular mechanism of BSQJ in regulating AS ossifification.</p>

A prospective study of heat sensitive moxibustion combined with Bushen Huoxue herbs in the treatment of sterility due to ovulatory dysfunction / 中国生化药物杂志

Objective To investigate the effect of heat sensitive moxibustion combined with Bushen Huoxue traditional Chinese medicine for treating sterility due to ovulatory dysfunction, provide evidence-based medicine proof for clinical treatment of ovulation dysfunction infertility optimization scheme.Methods This was a prospective, randomized, parallel controlled study.The control group injected recombinant human chorionic gonadotrophin ( HCG) by intramuscular, the observation group used the traditional Chinese medicine heat sensitive moxibustion combined with Bushe Huoxue herbs therapy, with the total curative effect, the ovulation rate, pregnancy rate, B ultrasound follicle image as the main observation indexes to judge the effect of treatment.Results After treatment, the of control group and treatment group patients were able to ovulate; the total effective rate of treatment group was 100%, slightly higher than the control group, by Ridit analysis, R =0.523, P =0.720; after treatment, two groups of pregnancy success rate approaches, P=0.243, with no significant difference; the average healing time was shorter than that of control group (P<0.05); the diameter of the largest follicle increased in two groups after treatment, but the difference was not statistically significant;the treatment group improved the total efficiency of the kidney deficiency was higher than that in control group, but the difference was not statistically significant.Conclusion Application of combined treatment of Bushen Houxue herbs and heat sensitive moxibustion can improve the ovulation rate and pregnancy rate in patients of ovulation dysfunction infertility with kidney deficiency and blood stasis, convenient and economic.

Diagnosis Value of PCT Combined with Respiratory Pathogen Detection in Pneumonia in Children / 现代检验医学杂志

Objective To investigate the diagnosis value of serum procalcitonin(PCT)detection combined with antibody de-tection of respiratory pathogens in children with pneumonia.Methods 1 256 cases of pneumonia in children were collected in the hospital from 2013 July to 2014 January were analyzed,detection PCT by chemiluminescence,using enzyme linked immu-nosorbent assay for detection of Mycoplasma pneumoniae ,Chlamydia pneumoniae and common respiratory virus.Results The positive rates of PCT was 30.7%,and MP IgM,CP IgM,RSV IgM,ADV IgM and FLUA IgM were 21.4%,16.8%, 11.8%,8.4%,and 10.9%.PCT in children with different gender in the largest number of positive cases,respectively,male 227 cases and female 158 cases,but the PCT and respiratory pathogen antibody positive in different gender did not exist sta-tistical difference (P >0.05).In different age groups,procalcitonin positive cases in 1 years in the group most,Mycoplasma pneumoniae and Chlamydia pneumoniae IgM positive cases in 1~3 years old were the largest number,the number of respir-atory virus positive patients with different maximum performance in different age groups.PCT and respiratory pathogen an-tibody positive in different age there was no statistical difference (P >0.05).Conclusion The PCT combined with respira-tory pathogen detection of antibodies help the clinic to determine the type of pneumonia in children,to provide help for the diagnosis and treatment of pneumonia in children.

Effect observation on integrated intervention in diabetic eye disease in a community of Shanghai / 上海预防医学

[Objective] To explore and evaluate the mode of integrated intervention in diabetic eye disease by general service team . [ Methods ] Integrated intervention for prevention and treatment of blindness and health education were carried out by general service team through a 2-year prospective co-hort study .The evaluation was made with the indicators such as mastery of health knowledge , ability of dis-ease management , laboratory parameters change , vision improvement and changes of diabetic retinopathy . [ Results] It was found that 8 items on health knowledge , 6 items on ability of disease management , 3 laboratory parameters and condition in the control of visual impairment and diabetic retinopathy were all better in the intervention group than in the control group . [ Conclusion] The effect of integrated intervention mode is significant by general practitioners and specialists participating in chronic disease prevention network .

Status quo investigation on the use of summary procedure in sanitation administation punishment / 上海预防医学

[Objective ] To ascertain the current situation in application of summary procedure within the jurisdiction , and propose corresponding measures and suggestions for prevention of administrative reconsideration and administrative lawsuits resulting from improper use of summery procedure . [ Meth-ods] Retrospective examination was done of 63 administrative penalty cases by summary procedure in Xu-hui District in 2011 and 2012 .Some parties punished were visited , and at the same period were randomly selected the penalty cases from the same professional use and general procedure .Control analysis was done from six aspects such as punishment body identified , confirmation of illegal facts , fixation of evidence , ap-plication of legal provisions , legitimate punishment procedure , clerical production specification and others . [ Results] Problems were found to be existent in the six aspects of punishment body identified , confir-mation of illegal facts , fixation of evidence , application of law provisions , legitimate punishment proce-dures, and clerical production specification , etc.There is a certain gap with the general procedure punish-ment cases , which needs to be improved in future . [ Conclusion ] Administration by law awareness should be strengthened , training in this regard enhanced , management norms for summary procedure formu-lated , evidence system for summary procedure established and perfected , and inspection strengthened , so as to improve the quality of summary punishment cases .

The roles of PPAR-gamma/PGC-1alpha to Nrf2/gamma-GCS-h in lung of guinea pigs with bronchial asthma / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h in lung of guinea pigs with bronchial asthma, and to explore the roles of them.</p><p><b>METHODS</b>Forty adult male guinea pigs were randomly divided into 4 groups: the control group (group A), asthmatic group ( group B), dexamethasone group (group C) and rogridone group (group D), 10 guinea pigs in each group. The asthmatic model was established by the ovalbumin challenge method. Expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h mRNA in lung tissue were assayed by in situ hybridization. Expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h protein were detected by immunohischemistry and by Western blot.</p><p><b>RESULTS</b>In situ hybridization showed that the expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h mRNA in lung tissue were the lowest in group B and the comparison among groups showed statistical significant (all P < 0.01). Immunohistochemistry and Western blot indicated that the value of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h protein in lung tissue were the lowest in group B, and expressed primarily in nucleus, the differences being statistically significant (all P < 0.01). There was positive correlation between PPAR-gamma and PGC-1. gamma-GCS-h mRNA also positively correlated between PPAR-gamma/PGC-1alpha and Nrf2 in nucleus, and the expression of Nrf2 was also positively correlated with PPAR-gamma/ PGC-1alpha.</p><p><b>CONCLUSION</b>In acute asthmatic models induced by ovalbumin, the expressions of PPAR-alpha/PGC-1alpha and Nrf2/gamma-GCS-h were decreased, and PPARgamma/PGC-1alpha could up-regulate the expressions of Nrf2/gamma-GCS-h to increase the antioxidant defense of tissues, thus being implicated that PPARgamma/PGC-1alpha might play important roles in the pathogenesis and prevention of asthma.</p>

Sex hormones in female patients with systemic lupus erythematosus / 中南大学学报(医学版)

OBJECTIVE@#To explore the levels of sex hormones in female patients with systemic lupus erythematosus (SLE) and its role in the genesis of SLE.@*METHODS@#The serum levels of estradiol, estriol, testosterone, progesterone, and prolactin were determined by electro-chemiluminescence immunoassay in 98 female patients with SLE and compared with those of 38 healthy women.@*RESULTS@#The serum levels of estradiol, estriol, progesterone, and prolactin were significantly higher than those in the healthy women (P <0.05). The serum levels of estradiol, progesterone, and prolactin in patients with SLE in 25 to 34 year old group were higher than the other age groups and the control group (P < 0.05), and the serum levels of estradiol and prolactin in patients with active phase of SLE were significantly higher than those in patients with stable phase of SLE (P <0.05).@*CONCLUSION@#The levels of sex hormones have a close corretation with the genesis and development of SLE.

Obesity and diseases in the elderly inpatient / 中南大学学报(医学版)

OBJECTIVE@#To explore the obesity distribution in old people and the relation between senile obesity and health.@*METHODS@#First, a questionnaire was designed which included chronic disease history, body mass index (BMI), physiological value, biochemistry index, anti-oxidation index, diagnosis of diseases, etc. Second, the measure and detection methods were unified; and the last, the investigation was made along with daily clinical work by clinicians.@*RESULTS@#We received 391 questionnaires. The overweight rate was 36.1% and the obesity rate was 7.9% . Total anti-oxidation activity in serum (TAS) and superoxide dismutase (SOD) decreased with body mass index (BMI), and the value in the obesity group was the lowest; Malonaldehyde (MDA) of overweight obesity was the largest. The mean blood pressure, blood fat, and blood glucose as well as the prevalence of cardiovascular disease, hyperlipemia, and glycuresis increased with BMI; and the value in the obesity group was the largest.@*CONCLUSION@#The prevalence of the senile obesity was below the average and the senile obesity complications were various and serious, and perhaps related to imbalance of free radical's production and cleanup, so the senile obesity seriously harmed old people's health.

STUDY ON CONDITIONS OF SWINE BLOOD MEAL FERMENTATION WITH COMPOUND STRAINS / 微生物学通报

Two high protease producing strains, whic h are desi gnated Aspergillus oryzae AS100 and AS102, were screened and applied as main fermentation strains of swine blood meal. A Saccharomyces cerevisiae Y113 and a Bacillus Asp007 were also used as assistant fermentation strains. The enzyme production abilities we re studied,and a series of parameters of the fermentation technology were deter mined. Through swine blood meal fermentation, a high-protein fermented feed wa s produced, which is strong soy flavor and was rich in protein(69%), free amino acid, vitamin such as VitD 3 and niacin and organic elements such as Fe. It is a kind of high-protein feed for animals and it could be used as feedstuff addi tive.