Herpes simplex virus-mediated RNA interference targeting vesicular glutamate transporter 3 attenuates tactile allodynia in mice / 南方医科大学学报

<p><b>OBJECTIVE</b>To construct a replication-deficient herpes simplex virus (HSV-1) for delivering a short hairpin RNA (shRNA) targeting vesicular glutamate transporter 3 (VGLUT3) and observe its effect in alleviating allodynia in mice.</p><p><b>METHODS</b>The recombinant HSV-1 vector carrying the shRNA targeting Vglut3 (HSV-1-shvglut3) was constructed and inoculated in the sciatic nerve in a mouse model of mechanical allodynia to test its analgesia effect. Mechanical allodynia and heat hypersensitivity of the mice were tested by von Frey filaments and Hargreaves' test, respectively. VGLUT3 expression in the dorsal root ganglion (DRG) was evaluated by immunohistochemistry and Western blotting.</p><p><b>RESULTS</b>Following inoculation in the sciatic nerve, the HSV vector HSV-1-shvglut3 was retrogradely transported to the DRG. Mechanical withdraw thresholds of the mouse models receiving HSV-1-shvglut3 inoculation were reversed to nearly the baseline level, and VGLUT3 expression in the DRG was down-regulated 2 weeks after vector inoculation. The analgesic effect lasted for over 2 weeks in these mice without obvious systematic side effects or changes in heat hypersensitivity threshold.</p><p><b>CONCLUSION</b>Vglut3 in the DRG is a promising therapeutic target for alleviating mechanical allodynia, and HSV-1 vector-mediated RNA interference is safe and efficient for inducing long-lasting analgesia after peripheral inoculation of the vector.</p>

Phosphoproteomics and its application in cancer research / 中南大学学报(医学版)

Phosphoproteome is the whole complement of phosphorylated proteins in a cell, tissue or organism, and has become an interesting study subject since the discovery of phosphorylation as a key regulatory mechanism of cell life. Phosphoproteomics is a method which studies the compact of the phosphorylated proteins, expression and modification, interaction and association, rule of the regulatory and so on. Recently, phosphoproteomics is widely used in cancer research. It will provide important information in cancer research, cancer diagnosis, and therapy.

The Correlation between Cognitive Functions and MR Volumetric Measurements of Medial Temporal Lobe in Mild Cognitive Impairment and Different-phase Alzheimer Disease / 中国心理卫生杂志

0.05).Attention,ability of calculation(MMSE items)and DS scores were negatively correlated with left amygdala volumes(r=-0.51～-0.57,P