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1.
West China Journal of Stomatology ; (6): 229-235, 2019.
Artículo en Chino | WPRIM | ID: wpr-772670

RESUMEN

Tooth preparation is the primary and core operation technique for dental esthetic restoration treatment, due to its effect of providing restoration space, bonding interfaces and marginal lines for dental rehabilitation after tooth tissue reduction. The concept of microscopic minimal invasive dentistry put forward the issue of conducting high-quality tooth preparation, conserve tooth-structure, protect vital pulp and periodontal tissue simultaneously. This study reviewed the concepts, physiology background, design and minimal invasive microscopic tooth preparation, and in the meantime, individualized strategies and the two core elements of tooth preparation (quantity and shape) are listed.


Asunto(s)
Porcelana Dental , Restauración Dental Permanente , Estética Dental , Preparación del Diente
2.
International Journal of Oral Science ; (4): 233-240, 2014.
Artículo en Inglés | WPRIM | ID: wpr-290172

RESUMEN

Chlorogenic acid (5-caffeoylquinic acid, CGA) is a phenolic compound that is found ubiquitously in plants, fruits and vegetables and is formed via the esterification of caffeic acid and quinic acid. In addition to its notable biological functions against cardiovascular diseases, type-2 diabetes and inflammatory conditions, CGA was recently hypothesized to be an alternative for the treatment of neurological diseases such as Alzheimer's disease and neuropathic pain disorders. However, its mechanism of action is unclear. Voltage-gated potassium channel (Kv) is a crucial factor in the electro-physiological processes of sensory neurons. Kv has also been identified as a potential therapeutic target for inflammation and neuropathic pain disorders. In this study, we analysed the effects of CGA on the two main subtypes of Kv in trigeminal ganglion neurons, namely, the IK,A and IK,V channels. Trigeminal ganglion (TRG) neurons were acutely disassociated from the rat TRG, and two different doses of CGA (0.2 and 1 mmol⋅L(-1)) were applied to the cells. Whole-cell patch-clamp recordings were performed to observe alterations in the activation and inactivation properties of the IK,A and IK,V channels. The results demonstrated that 0.2 mmol⋅L(-1) CGA decreased the peak current density of IK,A. Both 0.2 mmol⋅L(-1) and 1 mmol⋅L(-1) CGA also caused a significant reduction in the activation and inactivation thresholds of IK,A and IK,V. CGA exhibited a strong effect on the activation and inactivation velocities of IK,A and IK,V. These findings provide novel evidence explaining the biological effects of CGA, especially regarding its neurological effects.


Asunto(s)
Animales , Ratas , Analgésicos , Farmacología , Animales Recién Nacidos , Técnicas de Cultivo de Célula , Ácido Clorogénico , Farmacología , Activación del Canal Iónico , Potenciales de la Membrana , Neuronas , Neurotransmisores , Farmacología , Técnicas de Placa-Clamp , Canales de Potasio con Entrada de Voltaje , Ratas Sprague-Dawley , Ganglio del Trigémino
3.
International Journal of Oral Science ; (4): 117-124, 2011.
Artículo en Inglés | WPRIM | ID: wpr-269668

RESUMEN

When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells. DFAT cells re-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells are introduced and the current profiles of their cellular nature are summarized. Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials. In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine.


Asunto(s)
Animales , Humanos , Adipocitos , Fisiología , Células Madre Adultas , Técnicas de Cultivo de Célula , Desdiferenciación Celular , Linaje de la Célula , Perfilación de la Expresión Génica , Células Madre Mesenquimatosas , Células Madre Multipotentes , Neovascularización Fisiológica , Pericitos , Biología Celular , Ingeniería de Tejidos , Métodos
4.
Chinese Journal of Stomatology ; (12): 425-430, 2011.
Artículo en Chino | WPRIM | ID: wpr-306417

RESUMEN

<p><b>OBJECTIVE</b>To establish an animal model of titanium implant and to investigate the effects of low intensity pulsed ultrasound (LIPUS) on the implant osseointegration.</p><p><b>METHODS</b>Twenty-four female Sprague Dawley (SD) rats were used. Titanium implants were inserted into tibia metaphysis bilaterally in each SD rat. Since the second day following implant surgery, LIPUS stimulation were carried out around the implant on the right side for 20 minutes per day, and implants on the left side remain untouched as self-control. Eight rats were sacrificed and tibiae specimens were harvested on the end of the 4th, 8th, and 12th week respectively. X-ray picture, micro-CT analysis and histological sections were performed to evaluate the effects of LIPUS on the implant osseointegration.</p><p><b>RESULTS</b>Osseointegration at the interface between the implant and bone tissue was observed bilaterally. In the micro-CT histomorphometry analysis, the percentage of osseointegration (%OI) of experiment side were (45.708 ± 3.316)%, (46.231 ± 1.954)%, and (46.807 ± 1.451)% on the end of the 4th, 8th, and 12th week. Meanwhile, the %OI of control side were (43.021 ± 3.558)%, (44.272 ± 3.023)%, and (44.894 ± 4.215)% respectively. The %OI on the 4th weekend, bone volume ratio (BV/TV) on the 4th and 8th weekend, trabecular thickness (Tb. Th) and degree of anisotropy (DA) on the 8th and 12th weekend of the experiment side tibia were significantly greater than those on the control side (P < 0.05). The histological examination showed that the thickness of new bone around the implant and the bone-implant contact area was obviously greater in the LIPUS treated side compared to the control side.</p><p><b>CONCLUSIONS</b>The LIPUS therapy may accelerate the bone healing and osseointegration at the interface between titanium implant and bone, and promote remodeling of bone trabecula on the early stage.</p>


Asunto(s)
Animales , Femenino , Ratas , Fenómenos Biomecánicos , Densidad Ósea , Implantes Experimentales , Oseointegración , Distribución Aleatoria , Ratas Sprague-Dawley , Tibia , Cirugía General , Titanio , Química , Terapia por Ultrasonido , Métodos , Microtomografía por Rayos X , Métodos
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