Advanced natural products chemistry research in China between 2015 and 2017 / 中国天然药物

In this review, we intensively focus on the advances in research of natural products (NPs) discovery carried out by domestic scholars in China from 2015 through 2017. In general, a total of 1811 publications (1479 in English and 332 in Chinese) were accumulated regarding newly isolated NPs from plants, microorganisms, and marine sources. As a result, 277 selected papers concerning naturally occurring compounds with extraordinary frameworks, origins, and promising activities were discussed in this review article, mainly organized according to their structural classes and novelties.

Advanced natural products chemistry research in China between 2015 and 2017 / 中国天然药物

In this review, we intensively focus on the advances in research of natural products (NPs) discovery carried out by domestic scholars in China from 2015 through 2017. In general, a total of 1811 publications (1479 in English and 332 in Chinese) were accumulated regarding newly isolated NPs from plants, microorganisms, and marine sources. As a result, 277 selected papers concerning naturally occurring compounds with extraordinary frameworks, origins, and promising activities were discussed in this review article, mainly organized according to their structural classes and novelties.

An analysis on the trend of thyroid cancer incidence among urban residents in Hangzhou City / 预防医学

Objective To learn the epidemic situation of thyroid cancer in Shangcheng District of Hangzhou City and to explore the influencing factors.Methods The data of thyroid cancer incidence from 2006 to 2014 was collected and the annual incidence rates were adjusted by Segi's world standardized population.Results The average incidence of thyroid cancer was 25.48 /100 000 and was 28.28 /100 000 when adjusted by Segi's world standardized population.The incidence of thyroid cancer among female was 2.56 times higher than that among males.The incidence of female thyroid cancer was higher than male,and the incidence of 30 -50 year old group was highest.The incidence of thyroid cancer was increasing year by year.Conclusion The incidence of thyroid cancer in Shangcheng District of Hangzhou was higher than that in China and world,and it was increasing year by year.Thyroid cancer has been the major public health problem,and the government should do harder work to decrease the incidence of thyroid cancer.

Neuroprotective effect screening and the mechanism of 10 kinds of coumarin derivatives / 药学学报

The study reports the detection of neuroprotective effect of 10 kinds of coumarin derivatives and explores their possible mechanism. MTT method was used to screen the neuroprotective effect of 10 coumarin derivatives on neurotoxic agents (Aβ25-35 and rotenone) or OGD (oxygen-glucose deprivation). A compound with better protective effect was obtained. Then the effect of this compound on neurotoxic agents on PC12 was detected by the morphological observation. Furthermore, the effect of compound 3 on microglia with lipopolysaccharide (LPS) induced inflammation was detected. And the inflammatory factor was tested. Finally, direct free radical scavenging ability was detected. Compound 3 was found to be the best compound through three neurons toxic models. Not only compound 3 ameliorated cell viability reduced by three neurons toxic models, but also significantly inhibited the production of inflammatory factor (TNF-α and IL-1β). And its free radical scavenging ability is very good, especially the effect on superoxide anion, which is comparable with vitamin C. The significant scavenging effect of compound 3 on superoxide anion might be the mechanism of the neuroprotection. Compound 3 as a potential neural cell protective agent merits further investigation.

A dynamic investigation of smoking -related knowledge, attitude and behavioral among adult residents in Shangcheng District / 预防医学

Objective To evaluate the changes of smoking related knowledge,attitude and behavior among adult residents and to provide evidence for tobacco control strategy.Methods The adult residents in Shangcheng District were investigated by Multi -stage stratified radom sampling method in 2007,2010 and 2013,and the dynamic status of knowledge,attitude and behavior of smoking were analyzed.Results There were 239,250 and 259 people investigated in 2007,2010,and 2013,respectively.The current smoking rates of the interviewees were 25.52%,22.40%,and 24.71%,and there was no changes during these years(P >0.05).The awareness rates of bronchitis and lung cancer were more than 90%,and the awareness rates of lung cancer increased by years(P <0.05).Family smoking behavior rate decreased from 49.37% to 35.14%.The passive smoking rate decreased from 63.60% to 53.28% (P <0.05 ).The supporting rate of smoking prohibition at 7 public places increased (P <0.05),with the entertainment venues from 71.55% to 84.17% and school, hospital from 87.87% to 98.07%.Conclusion Family smoking rate and passive smoking rate decreased.The supporting rate of smoking prohibition at common area is increasing.

Research and application of absorbable screw in orthopedics: a clinical review comparing PDLLA screw with metal screw in patients with simple medial malleolus fracture / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To observe the therapeutic effect of absorbable screw in medial malleolus fracture and discuss its clinical application in orthopedics.</p><p><b>METHODS</b>A total of 129 patients with simple medial malleolus fracture were studied. Among them, 64 patients were treated with poly-D, L-lactic acid (PDLLA) absorbable screws, while the others were treated with metal screws. All the patients were followed up for 12-20 months (averaged 18.4 months) and the therapeutic effect was evaluated according to the American Orthopaedic Foot and Ankle Society clinical rating systems.</p><p><b>RESULTS</b>In absorbable screw group, we obtained excellent and good results in 62 cases (96.88%); in steel screw group, 61 cases (93.85%) achieved excellent and good results. There was no significant difference between the two groups.</p><p><b>CONCLUSION</b>In the treatment of malleolus fracture, absorbable screw can achieve the same result compared with metal screw fixation. Absorbable screw is preferred due to its advantages of safety, cleanliness and avoiding the removal procedure associated with metallic implants.</p>

Oil of Piper longum unsaponifiable matter prevents cholesterol gallstone formation / 药学学报

To observe the effect of various doses of oil of Piper longum unsaponifiable matter (OPUM) to cholesterol gallstones in experimental mice. C57BL/6 mice (n = 60) were randomly divided into 6 groups: control group, model group, OPUM (15, 30 and 60 mg x kg(-1)) group and ursodeoxycholic acid (UDCA, 60 mg x kg(-1)) group, administered for 10 weeks. The level of serum lipid and liver function enzymes were tested. The gallbladder was removed and bile was obtained by centrifugation. Next, the levels of the bile total cholesterol (TC), phospholipid (PL) and bile acid (TBA) were measured. The indicators of lipid peroxidation were determined and cholesterol saturation index (CSI) was calculated. The liver histological changes were observed by HE staining. The results showed that serum TC, TG (triglycerides) and AST (aspartate transaminase) contents, gallbladder cholesterol crystallization and CSI increased significantly (P < 0.05). In addition, the activity of SOD decreased significantly and MDA content increased significantly in liver (P < 0.05). HE staining results showed that the hepatic cord disorder and intracellular lipid droplets increased significantly. All results indicate that lithogenic diet lead to the formation of cholesterol gallstones. In OPUM (30 and 60 mg x kg(-1)) group, serum TC, TG and AST content, gallbladder cholesterol crystallization and CSI decreased significantly, the activity of SOD increased significantly and MDA content decreased significantly. HE staining results showed that OPUM can improve the morphology of liver cell, reduce the degree of hepatic cord disorders and restore the cell morphology close to normal. The cause of OPUM prevents cholesterol gallstone formation maybe due to protect the integrity of the liver cells, lower CSI, and reduce cholesterol crystal formation and hence prevent cholesterol gallstone formation.

Advances in the study of small molecule antagonists of chemokine receptors as anti-asthma agents / 药学学报

Asthma is a chronic inflammatory respiratory disease accompanied with airway inflammation, airway remodeling and bronchial hyperresponsiveness. Chemokines are important for the recruitment of immune cells to the lung, which play an important role in the formation and development of asthma. Targeting the chemokine receptors to anti-inflammation and anti-asthma is a new strategy and some candidate drugs are discovered recently. This review is focused on the development of chemokine receptor antagonists for anti-asthma, which will promote the compound designations.

MPP+ decreased BDNF expression in PC12 cells / 药学学报

The aim of this study is to investigate the neurotoxic effect and mechanism of 1-methyl-4-phenylpyridinium (MPP+) on PC12 cells. MTT assay was used to investigate cell viability, Western blotting assay was performed to observe the protein level and phosphorylation, and dual-luciferase assay was used to study the transactivation. The experiment showed that MPP+ could decrease cell viability significantly in a dose-dependent manner and could decrease BDNF protein level, depress the phosphorylation of ERK, and attenuate the phosphorylation and transactivation of CREB, which is one of transcription factors of BDNF, but did not affect the activity of CaMK II in PC12 cells. So MPP+ might decrease BDNF protein level through MAPK/ERK signal pathway.

Comparison of the action of isolichenin and methanol extract of saffron on long-term potentiation in hippocampal dentate gyrus in vivo / 药学学报

Long-term potentiation (LTP) is thought as a generative mechanism underlying learning and memory via storing information in central nervous system. Electro-neurophysiological assay for LTP is generally used in screening the drugs that can facilitate learning and memory. By using in vivo LTP technique, isolichenin was found to facilitate LTP induction by a tetanic stimulation (20 pulses/100 Hz) in dentate gyrus. This tetanic stimulation by itself, however, cannot induce LTP. Previous study showed the reagent being able to facilitate LTP-induction, like methanol extract of saffron (MES), usually can antagonize the inhibiting effect of 30% ethanol on LTP induction (30 pulses/60 Hz). Isolichenin may also fall into such kind of drugs. Interestingly, comparatively study showed that isolichenin failed to antagonize the inhibiting effect of 30% ethanol on LTP induction (30 pulses/60 Hz). This result indicates a different unknown mechanism existing in the effect of isolichenin on LTP or memory formation.

Comparison of the inhibitory activities of salvianolic acid B and Ginkgo biloba extract EGb 761 on neurotoxicity of beta-amyloid peptide / 药学学报

<p><b>AIM</b>To compare the effects of salvianolic acid B (Sal B) and Ginkgo biloba extract EGb 761 on beta-amyloid peptide (beta-AP) fibril formation and cytotoxicity to PC12 cells.</p><p><b>METHODS</b>The inhibitory effects of Sal B and EGb 761 on beta-AP1-40 fibril formation were determined by using fluorescence analysis with Thioflavin T (ThT) and electron microscopic image. beta-AP25-35 was aged by incubating at 37 degrees C for 7 d, then the protein was incubated with PC12 cells. The protective effects of Sal B and EGb 761 against cytotoxicity induced by aged beta-AP25-35 in PC12 cells were evaluated by MTT reduction assay and flow cytometric analysis. beta-AP25-35-induced accumulation of intracellular reactive oxygen species (ROS) was determined by fluorescence analysis.</p><p><b>RESULTS</b>Both Sal B and EGb 761 inhibited the formation of amyloid fibrils, protected PC12 cells from beta-AP25-35-induced cytotoxicity, and decreased ROS accumulation caused by beta-AP25-35. The effective doses of Sal B were far lower than those of EGb 761.</p><p><b>CONCLUSION</b>Sal B was much more efficient than EGb 761 in inhibiting beta-AP aggregation and in protecting PC12 cells from beta-AP-induced cytotoxicity.</p>

The mechanism of ginsenosides Rg1 and Rb1 promoting glutamic acid release from PC12 cells / 药学学报

<p><b>AIM</b>To study the mechanism of ginsenosides Rg1 and Rb1 promoting glutamic acid release from PC12 cells.</p><p><b>METHODS</b>The amount of glutamic acid released from PC12 cells was measured by high performance liquid chromatography (HPLC). The effect of Rg1 and Rb1 on the phosphorylation of synapsins was detected with immunofluorescent staining and Western blotting.</p><p><b>RESULTS</b>Both Rg1 (10 micromol x L(-1)) and Rb1 (10 micromol x L(-1)) increased glutamic acid release from PC12 cells. The release of glutamic acid was decreased by pre-incubating with the PKA inhibitor H89. H89 inhibited the release of glutamic acid induced by Rb1, but had no effect on the release of glutamic acid induced by Rg1. Moreover, Rb1 enhanced the phosphorylation of synapsins via PKA pathway, Rg1 was out of touch with this.</p><p><b>CONCLUSION</b>Rb1 may promote release of neurotransmitters by increasing the phosphorylation of synapsins via PKA pathway, whereas the up-regulation of neurotransmitters release induced by Rg1 is independent of the phosphorylation of synapsins.</p>

Study on the shu-points of five zang (viscera) for treatment of insomnia based on the theory of the five zang (viscera) storing vitality / 中国针灸

According to Prof. DONG Gui-rong's law of syndrome differentiation of "viscera, yin and yang, qi and blood, deficiency and excess" in internal injury diseases and academic thought of syndrome differentiation of regulating five zang vitality for treatment of insomnia, the author consults a great number of ancient and modern literature of TCM, analyzes the controlling functions of five zang storing vitality on whole organism and the relation between heart vitality and brain vitality, and explain the basis of acupuncture at five-zang shu-points of the Gallbladder Channel to regulate vitality of five zang-organs for treatment of insomnia from intension and functions of five zang vitality, whole regulative mechanism of five zang vitality, the cause and pathogenesis of insomnia of over or too less five zang-vitality induced by pathogenic factors, and according to typing and syndrome differentiation of five zang vitality, theory and principle of acupoint selection, so as to establish and put forward the method and theory of acupuncture at five zang shu-points of the Gallbladder Channel for treatment of insomnia.