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Objective To find out much more simply prepared, effective and abundant tissue slice dye. Methods Totally 27 kinds of directly used dress dye were tested to make mouse liver slice stained. Among them purple BL and blue B2R made hepatic structure including central vein and hepatic nucleus shown clearly. Then, the heart, kidney and testis slices of mouse were stained by purple BL and blue B2R. Results The solution preparation of Purple BL and blue B2R was just dissolved in distilled water in five minutes, so they were very much simple and time-saving.After the heart, kidney and testis slices of mouse were stained by purple BL and blue B2R,the stained tissue structure including cardiac muscle cross striation, kidney capsule cavity and spermatogenic cells is clear and similar to tissue stained by HE. Conclusion The effective dress dye purple BL and blue B2R are much more simply prepared and abundant than hematoxylin. So, it can be partly replaced by purple BL and blue B2R.
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<p><b>OBJECTIVE</b>To explore the inhibition mechanism and safety of pyrazolo[1,5-a]pyrazin-4(5H)-one derivatives against proliferation of human lung cancer A549 cells, H322 cells, and human umbilical vein endothelial cell (HUVEC).</p><p><b>METHODS</b>Cells were treated with 40 Μmol/L of the ppo3a, ppo3b, ppo3i, and 0.1% DMSO (control) for 48 hours, respectively. Apoptosis was determined by Hoechst 33258 staining assay in H322 and A549 cells. Cell cycle distribution was determined by flow cytometry analysis in A549 cell. LC3-II, p53, and heat shock protein (HSP) 70 protein levels were detected by Western blotting in A549 cells treated with ppo3b for 48 hours. The morphology and viability of HUVEC were observed by inverted microscope and sulforhodamine B (SRB) assay.</p><p><b>RESULTS</b>Ppo3a, ppo3b, and ppo3i significantly induced apoptosis in H322 and A549 cells. A strong G1-phase arrest was concomitant with the growth inhibitory effect on A549 cells. Ppo3b effectively elevated the p53 protein level, but significantly reduced the HSP70 protein level. There were no significantly inhibitory effect on the morphology and viability of HUVEC when treated with ppo3a, ppo3b, and ppo3i.</p><p><b>CONCLUSIONS</b>ppo3a, ppo3b, and ppo3i could inhibit H322 proliferation through apoptosis and inhibit A549 through apoptosis and G1-phase arrest. The protein p53 and HSP70 might involve in the inhibition effects. These derivatives might be a clue to find effective and safe drug for lung cancers.</p>