RESUMEN
<p><b>BACKGROUND</b>To construct human papillomavirus type 18 (HPV18 E6E7) adeno-associated virus (AAV) for studying the role of HPV E6E7 in the development of human cancer.</p><p><b>METHODS</b>HPV18 E6E7 genes were inserted into adeno-associated virus expression vector and then infected 293 cell line. The expression of HPV18 E6E7 genes were confirmed by using RT-PCR/Southern blot assay.</p><p><b>RESULTS</b>There was HPV18 E6E7 genes in the malignantly transformed cell line. The 293TL cells compared with the parent cells transformed cells grew more rapidly, lost their contact inhibition and formed more and large colonies in soft agar.</p><p><b>CONCLUSIONS</b>HPV18 E6E7 AAV was successfully constructed and could induce malignant transformation. HPV18 E6E7 AAV can be use for studying the immortalization and malignant transformation of human normal epithelial cells.</p>
Asunto(s)
Humanos , Línea Celular , Transformación Celular Neoplásica , Transformación Celular Viral , ADN Viral , Proteínas de Unión al ADN , Dependovirus , Genética , Células Epiteliales , Biología Celular , Virología , Feto , Riñón , Biología Celular , Proteínas Oncogénicas Virales , Genética , Papillomaviridae , Genética , Reacción en Cadena de la PolimerasaRESUMEN
The advancement of studies about the molecular biology and electronic physiology on sudden cardiac death was summarized in this article, including particularly cardiac concussion(commotio cordis), congenital long QT syndrome, and Brugada syndrome which probably resulting in fatal arrhythmia and sudden cardiac death. These corpses of fatal functional disorders often show the results of negative autopsy without obvious organic pathological changes. So when come across negative autopsy the medical examiner and the pathologist should be careful to investigate the inductive cause of sudden death, the history of disease, and the family history, then to rule out the possibility of the above disorders.