Toxic effect and mechanism of bis-benzylidine piperidon and proteasome inhibitor on tongue cancer cells / 中国药理学通报

Aim To investigate the toxic effects dibenzylidene piperidine RA190 and proteasome inhibitor MG132 on tongue cancer cell line CAL27 and TCA8113 and its mechanisms. Methods Different concentrations of RA190 or MG132 were exposed to tongue cancer cells for 24 hours. CCK-8 assay was used to detect the survival of CAL27 and TCA8113 cells, and flow cytometry was used to analyze the cell cycle and apoptosis of different groups of tongue cancer cells. Western blot was used to detect the expression of ADRM1, Cyclin B1, Bak and Bax protein in each group. Results The semi-inhibitory (IC