Analysis of chromosome aberrations in the cell derived from primary cell culture of laryngeal carcinoma and the Hep-2 cell line / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To search for characteristic chromosome changes in primary laryngeal squamous cell carcinoma (LSCC) and Hep-2 cell line and to realize the relationship between the cytogenetic abnormality and the pathogenetic mechanism in LSCC.</p><p><b>METHODS</b>The fresh resulted samples of LSCC were analyzed with an improved primary cell culture for chromosome preparation and G-banding technique. Hep-2 cell line was analyzed by high resolution banding technique. Molecular cytogenetics analysis was made by chromosome 6 painting probe.</p><p><b>RESULTS</b>Four primary LSCC succeeded in primary cell culture and obtained metaphases, one was tetraploid, the other three were triploid. The chromosome mode of Hep-2 cell line was from 68 to 75 and fifteen marker chromosomes were found. The most structural abnormalities of chromosome in primary LSCC and HEP-2 cell line were unbalance translocation, terminal deletion and isochromosome. The complicate aberration in chromosome 6 was common in LSCC and Hep-2.</p><p><b>CONCLUSION</b>6q-, I(5p), 17p-, 5q- are considered as characteristic chomosome changs in LSCC. Fluorescence in situ hybridization (FISH) may enhance the ability of detecting complicated chromosome rearrangements and marker chromosomes, which could provide more value data to verify the chromosome characteristic aberration in LSCC.</p>

Analysis of citations referenced in articles published in Chinese Journal of Integrated Traditional and Western Medicine from 2001 to 2004 / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the referential rule and the informative absorbing capacity of the Chinese Journal of Integrated Traditional and Western Medicine (CJITWM), and analyze the characteristics of literature requirement of scientists working in integrated Chinese and Western medicine (ICWM) field offering suggestions on literary utilization.</p><p><b>METHODS</b>Citation analysis was used to analyze the references cited in 1825 articles published in CJIM from 2001 to 2004 according to their time sequence of publishing.</p><p><b>RESULTS</b>The citation rate was 53.64%, and 9.51% citations per article. Most of the citations were mainly cited from journals (85.38%) and books (13.4%). The Price Index was 49.22%, and the self-citation rate for author and periodical were 3.63% and 7.77% respectively.</p><p><b>CONCLUSION</b>CJITWM is a highly authorized and representative professional academic periodical in the field of ICWM. The citations are mainly cited from periodicals written in Chinese or English, they are of good novelty and quality, but the citation rate should be further improved.</p>