Effect of a hypothetical gene Af116609 on multi-drug resistance of gastric cancer cells / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the effect of gene Af116609 on gastric cancer multi-drug resistance (MDR) by introducing it into gastric cancer multi-drug resistant (MDR) cell line SGC7901/VCR.</p><p><b>METHODS</b>Gene Af116609 was cloned from SGC7901/VCR by RT-PCR and its differential expression between gastric cancer MDR cells and its parental cells was displayed by Northern blot. The gene was introduced to gastric cancer cells by transfection of recombinant eukaryotic expression vector by electroporation. MTT assay in vitro was applied to investigate its effect on multi-drug resistance phenotype of gastric cancer cells.</p><p><b>RESULTS</b>The full length CDS of gene Af116609, as long as 327 bp, was cloned from gastric cancer MDR cell line SGC7901/VCR and its sequence was coincident with the hypothetical gene Af116609 in GenBank. It was overexpressed in MDR cells than its parental cells at mRNA level. In the MTT assay in vitro, the drug sensitive cells transfected with sense eukaryotic expression vector showed upregulated targeted gene, with increased resistance to vincristine, 5-fliorouracil and arabinoside, and decreased resistance to adriamycin, but no influence on resistance to methotrexate. However, the drug resistant cells transfected with anti-sense eukaryotic expression vector, showed down regulated targeted gene, with less resistance to all the five anticancer drugs to different degrees.</p><p><b>CONCLUSION</b>Gene Af116609 is related to MDR phenotype of gastric cancer cells and may become a candidate molecular target to reverse the MDR of gastric cancer.</p>

The expression and possible function of RhoA in human gastric cancer cell lines / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To study the expression and possible function of RhoA in human gastric cancer cell lines.</p><p><b>METHODS</b>The expression of RhoA in human gastrointestinal cancer cell lines was detected by Western blot. Antisense plasmid of RhoA was constructed by pGEFL and transferred into gastric cancer cell line AGS by lipofectamine. Cell survival was examined by MTT assays, and cell cycle was detected by flow cytometry.</p><p><b>RESULTS</b>The expression of RhoA protein in 10 different kinds of human cancer cell lines was much higher than that in immortalized human intestinal epithelial cell line. After being transfected with antisense RhoA, with the decrease in RhoA protein expression, the growth rate of AGS was inhibited, and the number of cells in S phase was increased by 14%.</p><p><b>CONCLUSION</b>RhoA is overexpressed in many human cancer cell lines. Some of the malignant characteristics of a gastric cancer cell line can be partially reversed by inhibiting RhoA expression.</p>

Differential expression of RPL6/Taxreb107 in drug resistant gastric cancer cell line SGC7901/ADR and its correlation with multiple-drug resistance / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the differential expression of RPL6/Taxreb107 between drug-resistant gastric cancer cell line SGC7901/ADR and gastric cancer cell line SGC7901 as well as its correlation with multiple-drug resistance (MDR) in gastric cancer cells.</p><p><b>METHODS</b>Total RNA was extracted from SGC7901 and SGC77901/ADR, with internal control RT-PCR, Northern blot, gene cloning and expression, construction of eukaryotic expression vector, gene transfection by electroporation. The accumulation and retention of ADR in transiently transfected cell was detected by flow cytometry.</p><p><b>RESULTS</b>The internal control RT-PCR and Northern blot showed high RPL6/Taxreb107 expression in SGC7901/ADR cell line. Sense and antisense eukaryonic expression vectors demonstrated by double enzyme digestion were successfully transfected into gastric cancer cell line SGC7901 and SGC7901/ADR respectively by electroporation. The accumulation and retention of ADR detected 48 hours after transfection showed that RPL6 gene had shown effect on drug resistance in gastric cancer cell.</p><p><b>CONCLUSION</b>The high expression of RPL6/Taxreb107 in drug resistant gastric cancer cell shows its correlation with multiple-drug resistance in gastric cancer.</p>

Protective effect of selective cyclooxygenase-2 inhibitor on alcohol-induced liver injury in rats / 中华肝脏病杂志

<p><b>OBJECTIVES</b>To investigate the effect of selective cyclooxygenase-2 (COX-2) inhibitor on alcohol-induced liver injury in rats.</p><p><b>METHODS</b>58 male Wistar rats were randomly divided into three groups: control group treated with dextrose and corn oil, model group with ethanol and corn oil, treatment group with corn oil and ethanol plus a selective COX-2 inhibitor celecoxib. All treatments were injected into stomach through intragastric tubes. Liver samples were analyzed for histopathology with light microscope (LM) and transmission electron microscope (TEM), and the expression of COX-2 with western blotting. Levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, levels of 6-Keto-prostaglandin F1 alpha (6-k-PGF1a) and thromboxane B2 (TXB2) in liver, and activity of glutathione s-transferase (GST) both in liver tissue and in plasma were measured.</p><p><b>RESULTS</b>LM and TEM indicated hepatocytes were injured obviously in the model group and slightly in the treatment group. The levels of AST and ALT in serum, TXB2 in liver and the activity of GST in plasma increased significantly in the model group (t> or =2.294, P<0.05), but the activity of GST in liver decreased significantly (t=8.856, P<0.01) compared with those in the control group. To compare with the model group, the levels of AST and TXB2 decreased significantly (t=4.305, P<0.01; t=2.799, P<0.01), meanwhile the activity of GST increased significantly (t=10.134, P<0.01) in the treatment group. COX-2 expression in liver by western blotting increased significantly in the model group, compared with the control group (t=4.067, P<0.01) and the treatment group (t=2.251, P<0.05). Exceptionally, the level of 6-k-PGF1a decreased significantly (t=2.284, P<0.05) in the model group.</p><p><b>CONCLUSION</b>COX-2 has involved in the alcohol-induced liver injury, and its inhibitor can diminish alcohol-induced liver injury in rats through decreasing TXB2 level</p>

Rac subfamily expression and activity in gastrointestinal cancer cell lines / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the significance of Rac subfamily members in the gastrointestinal carcinogenesis and progression.</p><p><b>METHODS</b>The mRNA expression of Rac1, Rac2 and Rac3 in 12 kinds of gastrointestinal cancer cell lines was examined by semi-quantitative RT-PCR. The activities of Rac1 protein in 5 kinds of gastric cancer cell lines were tested by pull-down assay.</p><p><b>RESULTS</b>Compared with the normal gastric mucosa and intestinal epithelial cell line, the mRNA expression of Rac1 and Rac3 was up-regulated in most of gastrointestinal cancer cell lines. The activities of Rac1 protein increased markedly in gastric cancer cell lines.</p><p><b>CONCLUSION</b>The increased mRNA expression of Rac1 and Rac3 in gastrointestinal cancer cell lines and the abnormal activation of Rac1 protein in gastric cancer cell lines might be correlated with the carcinogenesis of gastrointestinal cancer.</p>

Effects of different temperature and time on the quality of blood preservation in liquid blood / 中国应用生理学杂志

<p><b>AIM</b>To explore the effects of different temperature and time on preservation damages in liquid blood.</p><p><b>METHODS</b>Take blood sample from 10 healthy blood donors, put the fresh blood into CP2D-A liquid at 0 degrees C and 4 degrees C, and take the samples after 1 week, 2 weeks and 3 weeks, and then measured the contents of GSH-Px, TSH, LPO, the contracting protein of RBC, and membrane fluidity.</p><p><b>RESULTS</b>At the same temperature, when the preservation time is prolonged, peroxidation is increased, the preservation damages are also augmented; the damages are declined when the temperature is lower during the same period, the aging of blood was more evident at 4 degrees C.</p><p><b>CONCLUSION</b>Blood peroxidation temperature is lower. The 0 degrees C group is better than 4 degrees C group.</p>

Effects of different temperature and time on the period of validity and quality in blood preservation / 中国应用生理学杂志

<p><b>AIM</b>To examine the effects of different temperature protection on measures on preservation damages in liquid blood and explore the corresponding.</p><p><b>METHODS</b>Take equal half blood samples from 10 healthy blood donors and divided each sample into two groups, put the fresh blood into CP2D-A solution at 0 degrees C and 4 degrees C, respectively and take the samples 21 days and 42 days, later and then measured the contents of membrane phospholipids with shafig-UR-rehman method, CaM with purification PED test, LPO with spectrophotometry.</p><p><b>RESULTS</b>At the same temperature, when the preservation time was prolonged, peroxidation was increased, the preservation damages were also augmented; the damages were declined when the temperature was lower during the same period, the aging of blood was more evident at 4 degrees C.</p><p><b>CONCLUSION</b>Blood peroxidation temperature is lower. The author pointed out the questions and prospects of blood preservation.</p>