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Objective@#To explore the moderated and mediating mechanism between childhood trauma and depressive traits, and to provide a reference for conducting the related research and intervention.@*Methods@#From 2017 to 2018, a cluster sampling method was adopted to select 2 786 freshman. Childhood trauma questionnaire, state-trait depression Scale, the automatic thought questionnaire and trait coping style questionnaire was administered.@*Results@#Regression analysis showed that childhood trauma, automatic thinking and negative coping was positively associated with depressive traits, and positive coping was negatively associated with depressive traits (β=0.12, 0.40, 0.14, -0.33, P<0.05). The mediation analysis showed that automatic thinking mediated the relationship between childhood trauma and depressive traits (95%CI=0.03-0.06). The results of moderation effect showed that negative coping moderated the relationship between childhood trauma and automatic thinking and the relationship between childhood trauma and depressive traits, respectively (P<0.05).@*Conclusion@#Childhood trauma affects depressive traits,and its relationship is influenced by boping styles and automatic thinking.
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Objective To investigate the risk factors and treatments of invasive fungal infection (IFI) in recipients who underwent liver transplantation (LT).Methods We retrospectively analyzed the frequency and characteristics of IFI in 10 recipients who received LT between January 2005 and October 2018 in Tianjin First Central Hospital and evaluated the risk factors and treatments of IFI after LT.Results Of 10 cases of IFI after LT,9 cases obtained the definite diagnosis and one case clinical diagnosis.There was one case of mucor infection,4 cases of aspergillus infection (1 case was complicated with cryptococcus neoformans infection,2 cases of cryptococcus neoformans infection (one case was complicated with aspergillus infection),and 7 cases of candida infection.The survival rate of patients with IFI was significantly reduced.Liver failure,fungal colonization,indwelling catheter,concomtant gastrointestinal bleeding,intestinal dysfunction,and long-term application of broad-spectrum antibiotics were risk factors of IFI after LT.Conclusion Of 10 cases of IFI,the oceurance of infection in 9 cases is closely related to the preoperative status.Eetiology detection is difficult and takes long time.The biopsy is still the gold standard.High-throughput sequencing can be used as a supplementary means for microbiology detection.Preoperative assessment and early reasonable antifungal drugs are effective treatment measures for IFI after LT.
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The peptides,proteins and other biological molecules in transudative pleural effusion correlate directly or indirectly with specific physiological and pathological state,reflecting the information regarding the lungs or other parts of the body.In the present study,the peptide fraction in transudative pleural effusion was isolated by uhrafiltration.After desalted and enriched by C18 tips,the peptide mixture was analyzed by nano LC-MS/MS.The results showed that 314 peptides,which were originated from 52 proteins,in pleural transudate were identified.More than half of the peptides were derived from fibrinogen.Many peptides were characterized as displaying ladder sequences.In addition,a large number of proline oxidation modifications were detected in the peptides derived from collagen and fibrinogen.Gene ontology enrichment analysis showed that the most of the proteins extracellular properties of pleural transudate polypeptide components were protein with exocytosis.The study provided a rapid and efficient separation and analysis methods for lung disease markers related peptide compounds in pleural fluid leakage.Also this research provided a rapid and effective method for screening peptide biomarkers related to lung diseases from transudative pleural effusion.
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Abstract Formaldehyde has been widely employed to immobilize clinical tissue specimens, inactivate toxins and viruses in biomedical fields. Formaldehyde can react with active groups in bio-molecules such as proteins, resulting in protein cross-linking, inactivation, and immobilization. By using several standard peptides and tryptic peptides from matrix protein of influenza virus as experimental models, we studied the chemical modifications of peptides and proteins with formaldehyde by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and nano-electrospray quadruple time-of-flight tandem mass spectrometry. The reaction between formaldehyde and peptides was performed under the same conditions as those during inactivation of virus (4℃, 0. 025% Formalin (V/V), 37% formaldehyde solution (w/w), and 72 h). The results indicated that under above conditions, formaldehyde could react with amino group of N-terminus of standard peptide to generate a methylol adduct, which was further condensed into an imine to generate+12 Da product. Besides, formaldehyde could react with side chain of two amino acids such as arginine and lysine, yielding +12 Da product respectively. The analysis of the reaction between formaldehyde and tryptic peptides from matrix protein of influenza virus showed that +24 Da products could be detected in most peptides due to combinational contribution from N-terminus of peptide (+12 Da ) and side chain of C-terminal arginine or lysine (+12 Da) . Moreover, a +36 Da product was detected for a peptide with miss-cut site. The results indicated that low-concentration formaldehyde primarily reacted with amino group on N-termini of peptides and proteins, as well as the side chains of arginine and lysine residues. The present study suggested an effective mass spectrometry-based method for analyzing the reaction between low-concentration formaldehyde and peptides and proteins, thus provided strategies for interpretation for the mass spectra of reaction products.
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Interferon stimulated gene 15 kDa protein (ISG15) is the first ubiquitin-like protein identified, which plays vital roles in a variety of fields including viral infection and immunological regulation. In this study, liquid chromatography-tandem mass spectrometry was used to analyze ISG15-modified proteins in A549 cells in response to infection by influenza virus, which was enriched by immunoprecipitation. A total of 22 cellular host proteins were identified in A549 cells infected by influenza virus, including ubiquitin-like ISG15 protein, cyclin-T1, heat shock protein 71 kDa, caldesmon, eukaryotic translation initiation factor, and so on. Besides, non-structural protein (NS1) from influenza virus was also identified. Among the 22 host proteins identified, 6 proteins were also identified in the control non-infected A549 cells, including annexin A1, fructose-bisphosphate aldolase A, ATP synthase subunit g, enolase, actin, and tubulin. Bioinformatics analysis revealed that the identified ISG15-modified host proteins induced by influenza virus infection could be classified into 9 protein classes: chaperone, oxidoreductase, enzyme modulator, transferase, nucleic acid binding, transcription factor, kinase, cytoskeletal protein, and structural protein. This study provided a specific and effective tool for analyzing ISG15-modified proteins in proteome level.
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Objective To explore the effict of Jiegu-Qili tablet combined with intramedullary interlocking nails in the treatment of the fracture of tibia and fibula. Methods A total of 80 patients with fracture of tibia and fibula were selected, and were divided into two groups according to odd and even number of hospitalization. The surgery group (39 patients) was given intramedullary interlocking nails, the surgery and drug group (41 patients) was given Jiegu-Qili tablet combined with intramedullary interlocking nails. The perioperative parameters, the rate of fracture healing, knee scores and complications were evaluated during 12 months. Results The hospitalization (14.43 ± 2.47 d vs. 21.63 ± 3.16 d, t=11.385, P0.05). During 12 months follow-up, there were no statistical significance on complications between two group (7.3%vs.15.4%,χ2=1.303, P>0.05). Conclusions In summary, the Jiegu-Qili tablet combined with intramedullary interlocking nails had a good therapeutic effect on the fracture of tibia and fibula. It could increase the speed of fracture healing, shorten the hospitalization, reduce the postoperative pain and improve the activity of patients.
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<p><b>OBJECTIVE</b>To explore the inhibitory effect of alantolactone on the proliferation of adriamycin-resistant human chronic myelogenous leukemia cell line K562/ADR cells and its mechanism.</p><p><b>METHODS</b>K562/ADR cells were treated with various concentrations of alantolactone (0, 1, 2, 4, 6, 8, and 10 μmol/L) for different time points. Cell viability was analyzed with MTT assay. The effect of alantolactone on the apoptosis of K562/ADR cells was measured by flow cytometry. The expression of apoptosis-related proteins after treatment with alantolactone was analyzed using Western blot.</p><p><b>RESULTS</b>Alantolactone could effectively inhibit the proliferation of K562/ADR cells in dose- and time- dependent manner, the IC50 value of alantolactone treatment of K562/ADR cells for 24 h was 4.7 μmol/L (P<0.05). Flow cytometric analysis displayed that the apoptotic rates were 1.35%, 16.91%, 29.61% and 46.26%, respectively, after treatment with alantolactone at 0, 2.5, 5 and 7.5 μmol/L. Meanwhile, the expression of Bcl-2 and BCR-ABL proteins were significantly decreased and that of Bax, cytochrome C, cleaved-caspase-9, cleaved-caspase-3 and cleaved-PARP increased by alantolactone treatment.</p><p><b>CONCLUSION</b>Alantolactone had obvious inhibitory effect on the proliferation of K562/ADR cells through the caspase dependent mitochondrial(or intrinsic)apoptotic pathway.</p>