RESUMEN
Objective To study the effects of eicosapentaenoic acid (EPA) combined with etoposide on the proliferation and apoptosis of human lung cancer cell lines A-549 in vitro.Methods The A-549 cells were cultured,respectively,with culture fluids containing 40 μg/ml EPA,10 μg/ml etoposide,40 μg/ml EPA + 10 μg/ml etoposide,20 μg/ml etoposide,or 40 μg/ml EPA + 20 μg/ml.The effects of EPA and/or etoposide on the proliferation of the cell line were detected by MTT assay.The morphological changes of the cells were observed using annexin V-fluorescein isothiocyanate/propidium iodine and acridine orange/ethidium bromide double staining under fluorescence microscope.The apoptosis and the changes of cell cycle were detected by flow cytometry.Results The inhibition rates of EPA plus etoposide groups on the proliferation of human lung cancer cells A-549 were significantly higher than those in other etoposide groups (all P ＜ 0.05).Cell staining showed that the amount of apoptotic cells in EPA plus etoposide groups was significantly larger than that in etoposide groups.The percentages of cells in S and G2/M phases of EPA plus etoposide groups were significantly higher than those of etoposide groups (all P ＜ 0.05).Conclusion EPA may enhance the effect of etoposide on inhibiting proliferation and promoting apoptosis for human lung cancer cells A-549.
RESUMEN
Objective To investigate the effects of eicosapentaenoic acid (EPA) or EPA plus carboplatin on the proliferation and apoptosis of human lung cancer cell line A-549.Methods A-549 cells were cultured by 100 μg/ml carboplatin,80 μg/ml EPA,and their combination for 48 hours.MTT assay was used to determine the effect of EPA,carboplatin,or their combination on the proliferation of human lung cancer cell line A-549.The morphological changes of human lung cancer cell line A-549 were observed using HE staining,and apoptosis of A-549 cells was analyzed by flow cytometry.Results MTT assay showed that the proliferation inhibition rate of A-549 cells cultured with EPA plus carboplatin was 85.20％ ± 5.00％,which was significantly higher than that of cells cultured with 80 μg/ml EPA (32.85％ ± 3.00％,P =0.0001 ) or 100 μg/ml carboplatin (53.25％ ±3.00％,P =0.0013 ).HE staining showed apoptosis in all three groups,whereas the apoptosis rate reached 17.05％ ± 4.00％ in the combination group,which was significantly higher than that in carboplatin group (9.49％ ± 1.00％,P =0.0252).Conclusion EPA may enhance the effects of carboplatin in inhibiting the proliferation and promoting the apoptosis of human lung cancer cell line A-549.