RESUMEN
AIM: To observe the protective effect of composite salviae dropping pills against human umbilical vein endothelial cells(HUVECs) injure induced by hydrogen dioxide(H_2O_2) and discuss the mechanism of its therapeutic action on cardiac and encephalic diseases.METHODS: HUVECs were injured by 0.1 mmol/L H_2O_2 and then different final concentrations of composite salviae dropping pills(0.5 g/L,0.25 g/L,0.1 g/L) were added before and after the injury.Cell viability was measured by MTT assay.TBA method was used to detect the intracellular malonaldehyde. Nitric oxide(NO) in the culture medium was detected by using nitrate reductase assay,and immunocytochemisty was used to observe the expression of NOS2,NOS3 and NF-?B.Morphologic observation was also performed.RESULTS: HUVECs were injured by 0.1 mmol/L H_2O_2.Composite salviae dropping pills increased the cell viability apparently,inhibited the production of MDA induced by H_2O_2,regulated the generation of NO bilaterally and influenced the expression of NOS3 and NF-?B.CONCLUSIONS: Composite salviae dropping pills at concentrations of 0.5 g/L,0.25 g/L and 0.1 g/L protects HUVECs from injury by H_2O_2,no matter it is be added before or affter the injury.The possible mechanism is associated with its regulating the expression of NOS2,NOS3 and NF-?B.
RESUMEN
AIM: To observe the function and morphological change of human umbilical vein endothelial cells(HUVECs) treated with lipopolysaccharide(LPS) and composite salviae dropping pills(DSP).METHODS: HUVECs were cultured and incubated within 10 mg/L LPS for 12 hours.Different final concentrations of composite salviae dropping pills(1 g/L,0.5 g/L,0.25 g/L,0.1 g/L) were added before and after LPS treatment. Cell viability,NO,NOS,ET-1 and intracellular calcium were measured.The cells were observed under inverted microscope,inverted phase contrast microscope,laser confocal scanning microscopy and transmission electron microscope.RESULTS: When given after LPS treatment,different final concentrations of composite salviae dropping pills played a protective role(P0.05).The HUVECs injured by LPS underwent apparent morphological change after treatment with composite salviae dropping pills.CONCLUSION: Composite salviae dropping pills have an evident protective effect on human umbilical vein endothelial cells exposed to LPS.