Practice and discussion of scientific research reagent management in hospital / 中华医学科研管理杂志

Objective This research aims to improve the ability and level of the management of scientific research reagent in better support of hospital scientific research.Methods Establish a sound management system and build an information system for scientific research reagents,meanwhile summarize and optimize system functions in practice constantly.Results Scientific research reagent management work has been organized orderly and the procurement and use of scientific research reagents has been standardized in the hospital,which effectively reduced the purchase cost of scientific research reagents and ensured the smooth development of scientific research.At the same time,this manner provides researchers with a convenient and quality service as well as accelerates the scientific research process.Conclusions The perfect scientific research reagent management system can provide effective support and guarantee for the scientific research work of the hospital,which is conducive to the sustained and stable development of hospital scientific research.

Biocatalytic desymmetric hydrolysis of 3-(4-chlorophenyl)-glutaronitrile to the key precursor of optically pure baclofen / 生物工程学报

We produced (S)-4-cyano-3-(4-chlorophenyl)-butyrate by highly stereoselective biocatalyst in this study. A nitrilase-producing strain, named Gibberella intermedia WX12, was isolated by 3-(4-chlorophenyl)-glutaronitrile as substrate in the screening with phenol-sodium hypochlorite method. The fermentation conditions and catalytic properties of this strain were investigated. The preferred carbon and nitrogen sources for nitrilase production were lactose (30 g/L) and peptone (20 g/L). After being cultivated for 96 h, the cells were collected for use in biotransformation. The hydrolysis of 3-(4-chlorophenyl)-glutaronitrile was performed at 30 degrees C in phosphate buffer (pH 8.0, 50 mmol/L) for 24 h to give (S)-4-cyano-3-(4-chlorophenyl)-butyric acid with 90% yield and > 99% of ee, which can be used for the synthesis of (R)- and (S)-baclofen. The configuration of product was determined by chemically converting it to baclofen and comparison with the authentic sample by chiral HPLC analysis.