Expression of large tumor suppressor homolog 2 gene and its promotor methylation in oral squamous cell carcinoma / 中华口腔医学杂志

Objective@#To investigate the expression of large tumor suppressor homolog 2 (LATS2) gene and its promotor methylation in oral squamous cell carcinoma (OSCC).@*Methods@#Reverse transcription-PCR (RT-PCR) and pyrosequencing were used to detect the mRNA and promotor methylation of LATS2 gene in 72 OSCC specimens and normal oral mucosa tissues. Western blotting was used to detect the LATS2 protein in six OSCC specimens and normal oral mucosa tissues.@*Results@#All cases had expression of LATS2 mRNA in normal oral mucosa tissues, but the expression was down-regulated significantly, only 47% (34/72) in 72 cases of OSCC showed LATS2 mRNA expression. The expression was correlated with the degree of tumor differentiation and lymph node metastasis (P<0.05). The results of pyrosequencing show that 68% of promotor methylation (49/72) in 72 cases of OSCC. Furthermore, there was significant correlation between the mRNA and promotor methylation of LATS2 gene (χ2=16.980, P<0.01). All the six specimens had the low LATS2 protein expression.@*Conclusions@#The promotor methylation of LATS2 gene may play an important role in the occurrence of OSCC.

Expression and significance of COX-2 ,MMP-9 and PTEN in colonic adenocarcinoma tissues / 重庆医学

Objective To explore the expression and significance of COX-2 ,MMP-9 and PTEN in colonic adenocarcinoma tissues .Methods Seventy-two cases of pathologically archived paraffin specimens after colonic adenocarcinoma radical operation in the hospital from March 2014 to May 2016 were collected ,30 cases of colonic adenoma tissues and 30 cases of normal mucosal mem-brane tissues served as the controls .The expressions of COX-2 ,MMP-9 and PTEN were detected by adopting the immunohisto-chemical SP method .Then the correlation between COX-2 ,MMP-9 and PTEN with clinicopathological features of colonic adenocar-cinoma was analyzed .Results The positive expression rate of COX-2 and MMP-9 in colonic adenocarcinoma tissues was significant-ly higher than that in colonic adenoma tissues and normal colon mucosal membrane tissues (P<0 .05);the positive expression rate of PTEN was significantly lower than that in colonic adenoma tissues and normal colon mucosal membrane tissues (P<0 .05) .The expression of COX-2 ,MMP-9 and PTEN in colonic adenocarcinoma was correlated with the tumor invasive depth ,lymph node me-tastasis and TNM staging(P<0 .05) ,while had no correlation with sex ,age ,tumor morphology ,tumor size and differentiation de-gree(P>0 .05) .COX-2 ,MMP-9 and PTEN expressions showed negative correlation (r= -0 .260 ,-0 .282 ,P<0 .05) ,COX-2 and MMP-9 expression showed a positive correlation (r=0 .335 ,P=0 .004) .Conclusion The abnormal expression and interaction of COX-2 ,MMP-9 and PTEN are closely correlated with the occurrence ,invasion and metastasis of colonic adenocarcinoma .Their combined detection has an important significance for early diagnosis and prognosis assessment of colonic adenocarcinoma .

Correlation of LATS2 mRNA and protein expression in oral squamous cell carcinoma / 实用口腔医学杂志

RT-PCR and Western blot were used to detect the LATS2 mRNA and protein level in 57 cases of oral squamous cell carcinoma (OSCC)specimens and 12 normal oral mucosa tissues.LATS2 mRNA and protein were expressed in all the normal oral mucosa samples (100%),but only 47.4% (27 /57)and 42.1% (24 /57)in OSCC samples respectively with positive correlation of both(P <0.01),and were respectively correlated with the tumor differentiation degree and lymph node metastasis(P <0.05).

Induction effect of icotinib on apoptosis of salivary adenoid cystic carcinoma ACC-M cells through p38-MAPK pathway / 吉林大学学报(医学版)

Objective To explore the influence of icotinib in the apoptosis of the human salivary adenoid cystic carcinoma cells ACC-M, and to clarify the mechanism of icotinib for the treatment of salivary adenoid cystic carcinoma.Methods The ACC-M cells were randomly divided into control group,2,4,8μmo1·L-1 icotinib groups,p38-MAPK inhibitor SB203580 (20μmol· L-1 )group,SB203580 (20 μmol· L-1 )+4μmo1 · L-1 icotinib group;the cells were collected 4 h after treatment.The viability of ACC-M cells was measured by MTT assay.The apoptosis of ACC-M cells was assessed by caspase-3 activity kit. The expression of p-p38-MAPK protein was determined by Western blotting analysis.Results Compared with control group,the inhibitory rates of growth of the ACC-M cells in icotinib groups were significantly decreased (P<0.05 ), and the activities of caspase-3 were increased (P<0.05),and the expression levels of p-p38-MAPK were significantly increased (P<0.05).Compared with 4μmo1·L-1 icotinib group,the expression level of p-p38-MAPK in SB203580+icotinib group were decreased (P < 0.05 ), and the activity of caspase-3 was decreased dramatically (P < 0.05 ). Conclusion Icotinib may induce the apoptosis of ACC-M cells through the activation of p38-MAPK signaling pathway.

Research progress of PTEN pathway in anti-angiogenesis of tumors / 白血病·淋巴瘤

PTEN is a dual protein/lipid phosphatase.As a negative regulator of PI3K/Akt pathway,PTEN not only plays an important role in multiple cellular functions such as cell metabolism,proliferation,cell-cycle progression,but also modulates angiogenesis via down-regulating PI3K-Akt-VEGF-HIF pathway in many tumors.In this review,the recent studies on the roles and mechanisms of PTEN in regulating tumorigenesis and angiogenesis are discussed to explore PTEN as a favorable prospect target in the treatment of angiogenesis.

Proliferating activity and differentiation between peripheral cells and central cells in ameloblastoma / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the proliferating activities and differentiation between peripheral cells and central cells in ameloblastoma.</p><p><b>METHODS</b>Expressions of proliferating cell nuclear antigen (PCNA), Ki-67, CK10&13 and CK19 were detected in 43 ameloblastoma (15 follicular, 20 plexiform and 8 acanthomatous) by SP immunohistochemical methods.</p><p><b>RESULTS</b>The PCNA labelling indices were significantly higher in peripheral cells (5.12% +/- 2.76%) of tumour nests or strands than in central cells (1.36% +/- 1.02%, P < 0.001). The peripheral cells of tumour nests or strands exhibited a significantly higher Ki-67 labelling index (3.63% +/- 1.80%) than central cells (1.26% +/- 0.96%, P < 0.001). The labelling indices between PCNA and Ki-67 showed a significant correlation (P < 0.01). The positive expressions of CK10&13 and CK19 were significantly higher in central cells than in peripheral cells (P < 0.01).</p><p><b>CONCLUSIONS</b>The peripheral zones of tumour nests or strands are regarded as proliferating areas; there exists a significantly different differentiation between central cells and peripheral cells.</p>

A quantitatively pathological study on malignant ameloblastomas / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the role of nuclear morphometric parameters, DNA content and Ag-NOR count in the differentiating malignant and benign ameloblastomas.</p><p><b>METHODS</b>Totally 17 cases of malignant ameloblastomas were examined by using HE, AgNOR and DNA stain methods. Morphometric parameters of cell nuclei, DNA content and AgNOR count were quantitatively studied by using an image analysis system.</p><p><b>RESULTS</b>Seven parameters (area, perimeter, equal diameter, minor diameter, mean diameter, round index, axis ratio) out of ten shape factors were significantly different between malignant and benign ameloblastoma (P < 0.01). AgNOR count and DNA index in malignant ameloblastoma were significantly higher than those in benign ameloblastoma (P < 0.01). Logistic regression equation was established, according to nuclear morphormetric parameters and DNA index.</p><p><b>CONCLUSION</b>Quantitative analysis of DAN content, nuclear morphmetric parameters and AgNOR count may be helpful in differentiating malignant and benign ameloblastomas.</p>

Expressions of CD44v3 and CD54 in oral premalignant epithelia / 实用口腔医学杂志

Objective:To investigate the expressions of CD44v3 and CD54 in oral premalignant epithelia and its significance in the carcinogenesis of oral mucosa.Methods:The expressions of CD44v3 and CD54 in 19 cases of normal mucosa and mucosal hyperplasia,22 case of dysplastic epithelia,26 cases of oral cancer mucosa were detected by using immunohistochemistry.Results:CD44v3 and CD54 proteins showed no expression or micro-expression in normal and hyperplastic epithelia.The expressions increased in the dysplastic epithelia and mucosa of oral cancer gradually.The difference between the expressions of the three groups was significant(P

Proliferation and differentiation of malignant ameloblastoma / 实用口腔医学杂志

砄bjective:To study the characteristics of proliferation and differentiation of malignant ameloblastoma.Methods:The expression of PCNA,Ki 67, CK19 and CK10 and CK13 proteins in 17 cases of malignant ameloblastoma was detected immunohistochemically by SP method. Results: The proliferating indices of PCNA and Ki 67 were significantly higher in malignant ameloblastoma than those in benign ameloblastoma ( P