RESUMEN
AIM:To develop a HPLC method for determing the contents of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills(Radix et Rhizoma Salviae miltiorrhizae,Fructus Schisandrae chinensis,Rizoma Acori tatarinowii,etc.). METHODS: The samples were extracted with methanol through supersonic wave and then filtered.The determination was performed by HPLC on Shim-pack VP-ODS C_(18) column using methanol-water-acetic acid glacial(65∶35∶0.1) as a mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was at 220 nm. RESULTS: The linear ranges of schizandrol,schisantherin and deoxyschizandrin were 0.001 2-(0.050 0) ?g(r=1.000 0)、0.001 4-0.057 6 ?g(r=0.999 5) and 0.002 1-0.060 8 ?g(r=0.999 8),respectively.The average recoveries of three components were 99.51%,99.40% and 99.06%,respectively.(CONCLUSION:)The method is accurate,quick,sensitive and can be used for measurement of schizandrol,schisantherin and deoxyschizandrin in Anshen Buxin Pills.