RESUMEN
OBJECTIVE:To establish a RP-HPLC method for the determination of imperatorin in Qingwen jiedu tablets(heat-clearing and detoxifying agent).METHODS:The chromatographic separation was performed on Diamonsil-C18(250 mm?4.6 mm,5 ?m)with column temperature kept at 35 ℃.The mobile phase consisted of methanol-water(70∶30)at a flow rate of 0.8 mL?min-1 with detection wavelength set at 300 nm.RESULTS:The linear range of imperatorin was 2.7～27.0 ?g?mL-1(r=0.999 9)with its average recovery at 98.9%(RSD=1.44%,n=6).CONCLUSION:The established method was proved to be well-separated,sensitive,accurate and applicable for the quality control of Qingwen jiedu tablets.
RESUMEN
OBJECTIVE:To establish a capillary zone electrophoresis with high frequency conductance method for the determination of ursolic acid in Radix et Rhizoma Clematidis.METHODS:The separation of sample was performed on an uncoated fused silica capillary(60 cm?75 ?m ID,effective length:56 cm) with 1.2 mmol?L-1 triethylamine-HCl(pH 10.60) and 0.24 mmol?L-1 ?-cyclodextrin as running buffer solution.The separation voltage was 12.5 kV and the gravity injection of sample was conducted for 10 s at a height of 20 cm.RESULTS:The calibration curve of ursolic acid was linear over the concentration range of 5.55～111.0 mg?mL-1(r=0.999 3) and the average recovery for ursolic acid was 96.0%(RSD=1.39%,n=6). CONCLUSION:The method was proved to be simple,accurate,rapid and reproducible,and suitable for the quantitative determination of ursolic acid in Radix et Rhizoma Clematidis.