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J Biosci ; 2001 Dec; 26(5): 635-40
Artículo en Inglés | IMSEAR | ID: sea-111330

RESUMEN

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.


Asunto(s)
Western Blotting , Clonación Molecular , Concanavalina A/química , Fabaceae/química , Lectinas de Plantas , Precursores de Proteínas/química , Proteínas Recombinantes/química , Solubilidad
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