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Abstract Objective: to describe software performance in the automatic selection of the underlying cause of death in Peru, between 2016 and 2019. Methods: this was a descriptive study on the software performance in the automated selection of the underlying cause of death over the years (chi-square test for trend) and the correlation between the type of death certificate and software performance (correlation coefficient and coefficient of determination). Results: a total of 446,217 death certificates were analyzed; the proportion of death certificates with the underlying cause of death increased from 69.6% in 2016 to 78.8% in 2019 (p-value < 0.001); it could be seen a direct linear correlation between electronic death certificates and software performance (correlation coefficient = 0.95; R2 = 0.89). Conclusion: the software showed good performance in the automatic selection of the underlying cause of death, with a significant increase between 2016 and 2019.
Resumen Objetivo: describir el desempeño de un software en la selección automática de la causa básica de muerte en Perú, entre 2016 y 2019. Métodos: estudio descriptivo de la tendencia del desempeño de un software para seleccionar la causa básica de muerte a través de los años (chi cuadrado de tendencia) y la correlación entre los certificados de defunción electrónicos y el desempeño del software (coeficientes de correlación y determinación). Resultados: se analizaron 446.217 certificados; la proporción de certificados con causa básica de muerte aumentó de 69,6% en 2016 a 78,8% en 2019 (p-valor < 0,001); se observó una correlación lineal directa entre certificados electrónicos y el desempeño del software (coeficiente de correlación = 0,95; R2 = 0,89). Conclusión: el software presentó un buen desempeño en la selección de la causa básica de muerte y aumentó significativamente entre 2016 y 2019.
Resumo Objetivo: descrever o desempenho de um software na seleção automática da causa básica de morte no Peru, entre 2016 e 2019. Métodos: estudo descritivo do desempenho de um software na seleção automatizada da causa básica de morte ao longo dos anos (teste qui-quadrado de tendência) e da correlação entre a forma de declaração de óbito e o desempenho do software (coeficientes de correlação e determinação). Resultados: foram analisadas 446.217 declarações de óbito; a proporção de declarações com causa básica de morte aumentou de 69,6%, em 2016, para 78,8%, em 2019 (p-valor < 0,001); observou-se correlação linear direta entre as declarações eletrônicas de óbito e o desempenho do software (coeficiente de correlação = 0,95; R2 = 0,89). Conclusão: o software apresentou bom desempenho na seleção automática da causa básica de morte, com aumento significativo entre 2016 e 2019.
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Anaplasma and Ehrlichia species are important tick-borne pathogens that cause disease in cattle, dogs, horses and humans; with little information available about these agents in Colombia. The aim of this study was to provide molecular evidence for the presence of Anaplasma sp. and Ehrlichia sp. species in ticks collected from horses, dogs and cattle in Cordoba, Colombia. In this study, 1.105 ticks were removed from 226 zebu cattle (Bos indicus), 87 dogs (Canis lupus familiaris) and 19 horses (Equus caballus), from different localities of Cordoba. Ticks were identified taxonomically and PCR assays were used for the amplification of Anaplasma sp. and Ehrlichia sp. DNA. The amplification products were sequenced and analyzed. From the 1,105 ticks examined, 679 (61.5%) were Rhipicephalus microplus, collected from cattle, 353 (32%) were Rhipicephalus sanguineus from dogs and 73 (6,6%) Dermacentor nitens collected on horses; 332 pools were formed to develop the PCR assay. Anaplasma sp. and Ehrlichia sp. DNA was detected in 5.7% (19/332 pools). Direct sequencing of amplicons showed that seven sequences had similarities between 99–100% with Anaplasma marginale, one sequence showed 100% identical with Anaplasma phagocytophilum, seven sequences showed 100% identity with Ehrlichia ewingii, one sequence had 100% identity with E. chaffensis and three sequences showed similarities of 99% and 95% with Ehrlichia mineirensis and Ehrlichia canis respectively. In conclusion, several tick-borne pathogens identified in this survey suggests that there is a risk for the emergence of tick-borne diseases in domestic animals and humans in Colombia. Our data provides evidence of Ehrlichia and Anaplasma species circulating in ticks from Cordoba.
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A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geographic origin.
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Genotipo , Reacción en Cadena de la Polimerasa , Métodos , Técnica del ADN Polimorfo Amplificado Aleatorio , Métodos , Sequoia , Clasificación , GenéticaRESUMEN
Se realizó un estudio transversal, para evaluar algunos aspectos de la calidad de la atención médica brindada en los policlínicos principales de urgencia (PPU) del municipio 10 de Octubre. Para ello se aplicó un cuestionario autoadministrado a los usuarios seleccionados. La muestra quedó conformada por 328 pacientes. El comportamiento de los criterios seleccionados para medir la estructura y el proceso arrojó valores no aceptables en casi la totalidad de los criterios y manifestó amplias desviaciones de los valores obtenidos en relación con los establecidos. La calidad de estos servicios es susceptible de mejoras que permitan que estén a la altura de lo esperado por los usuarios y la resolutividad de sus problemas, la cual está determinada en gran medida por la falta de medicamentos en la farmacia. Se recomienda realizar estudios periódicos que permitan evaluar la calidad de la atención médica en los PPU del municipio 10 de octubre; con el fin de conocer cómo estos funcionan y si están en correspondencia con los objetivos para los que fueron creados