Rapid fluorescent focus inhibition test for rabies antibody estimation using murine neuroblastoma cell line.

A rapid fluorescent focus inhibition test (RFFIT) for rabies antibody estimation has been standardized in which murine neuroblastoma cell line, Neuro-2A and tissue culture microtiter plates were used. Serum samples from 105 human beings, 30 canines, 7 rabbits and 4 monkeys were tested by RFFIT. Of these, 33 human and 20 canine sera were also tested by the mouse neutralization test (MNT). Twelve human sera were tested by RFFIT, MNT and ELISA. There was 94 per cent correlation between the results obtained by RFFIT and MNT, while in 6 per cent of the sera tested, the RFFIT was found to be more sensitive than the MNT.