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Objective To observe the expression of inhibitor of Zeste 12(SUZ12) and matrix metalloproteinase-9(MMP-9) in retinoblastoma(RB) and their significance .Methods The paraffin embedded specimens from 56 patients with RB were studied ,10 patients belong to intraocular stage ,28 patients intraocular pressure increased stage and 18 patients outside ocular stage among them .22 ,16 ,12 ,and 6 patients with invasion degree(N) from N0-N3 differently ,while10 ,16 ,18 and 12 patients with cell differenti-ations(D) from D0-D3 differently .The expressions of SUZ12 and MMP-9 protein in 56 patient s were detected in different clinical stages ,invasion degree and cell differentiation by immunohistochemical staining and their correlations were analyzed .Results The expressions of SUZ12 and MMP-9 in RB tissues[(31 .48 ± 12 .20)% ,(34 .49 ± 13 .25)% ]were obviously higher than in para-cancer tissue[(0 .26% ± 0 .06)% ,(0 .36 ± 0 .08)% ](P<0 .01) .The expression of SUZ12 was related to clinical stages ,invasion degree and cell differentiation .The expression of SUZ12 and MMP-9 in intraocular pressure increased stage was obviously higher than in-traocular stage(t=10 .299 ,16 .253 ;P<0 .01) ,and outside ocular stage was obviously higher than intraocular stage (t= 20 .819 , 36 .417 ;P<0 .01)and intraocular pressure increased stage (t= 10 .348 ,15 .061 ;P< 0 .01) .The expression of SUZ12 and MMP-9 was increasing from N0 to N3 but decreasing from D0 to D3 .The expression of SUZ12 in RB was related to MMP-9 expression(r=0 .840 ,P=0 .000) .Conclusion The expression of SUZ12 and MMP-9 increased in RB tissue and it is helpful to predict the malig-nancy degree of RB .
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Objective To analyze the correlation between the expression of HIF-1α in multiple myeloma (MM) and clinical indexes in order to illustrate the expression and implication of HIF-1α gene in MM. Methods RQ-PCR method was used to amplify HIF-1α mRNA of bone marrow mononuclear cells isolated from 28 cases of MM patients and the control group. β-actin was used as internal standard. HIF-1α mRNA expression was analyzed by SDS software and the ratios of HIF-1α/ β-actin were calculated. Results The relative expression level of HIF-1α mRNA in MM bone marrow mononuclear cells was 12.68 times as that in control group. HIF-1α mRNA was positively correlated with β2-MG (r =0.575, P =0.000), ESR (r =0.522,P =0.000), LDH (r=0.286, P=0.044) and CRP (r =0.356, P =0.011). There was a negative correlation between HIF-1α mRNA and Hb (r =-0.556, P =0.000). Conclusion The expression of HIF-1α mRNA was up-regulated and HIF-1α was related to a number of clinical indexes. HIF-1α may be used to estimate the progress of MM and hopeful to be a new molecular target in cancer therapy.
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0.05) , but significant difference in latency time of N145 was found between the three groups (P