RESUMEN
Effects of melatonin ( MT) on Con A or LPS-induced prolifera-tive responses of splenocytes in mice and Con A induced interleukin 2 ( IL-2 ) production of splenocytes in rats were studied. The results showed that Con A and LPS induced proliferative responses of splenocytes were enhanced by in vitro treatment with MT (0.01~1?mol/L and 0.01~10?mol/L, reinduced-pectively ) . ConA IL-2 production of splenocytes were also enhanced by in vitro treatment with MT (1~10?mol/L), but MT has no effects oa above functions of splenocytes without activating by ConA or LPS. These data suggested that mitogen activated lymphocytes might be one of the target cells for MT.
RESUMEN
Effects of supernatants from cultured rat pineal glands on ConA and LPS induced proliferative responses of splenocytes in mice, ConA induced interleukin 2 (IL-2) production of splenocytes in rats were studied. These results showed that ConA and LPS induced proliferative responses of splenocytes were enhanced by supernatants from cultured rat pineal glands stimulated with 10 ?mol/L isoproterenol (ISO) for 60 min (Dilution. 1 : 45 ) , ConA induced IL-2 production of splenocytes in rats was also enhanced by the supernatants (Dilution: 1 : 135 ) .
RESUMEN
Effects of melatonin ( MT ) and supernatants from cultured pineal glands stimulated by 10 ?mol/L isoproterenol (ISO) for 0, 30, 60, 120 min on interleukin I ( IL-1 ) production by peritoneal macrophage ( PM? ) in rats were studied. Theses results showed that LPS-induced IL-1 production by PM? was enhanced by in vitro treatment with MT (0.1~1 ?mol/L ) , but IL-1 production was not influenced by MT without LPS. LPS-induced IL-1 production by PM? was enhanced in various degree by the supernatants, action of supernatants for 60min was best. The data is suggested that LPS-activated M?s may be one of target cells for MT.
RESUMEN
Effects of total glucosides of paeony ( TGP) on interleukin2 ( IL-2 ) production were studied with lL-2-dependent marine splenic lymohocytes. The results showed that IL-2 production by splenocytes was enhanced with low concentration of TGP (0.5-12.5 mg/L) , and diminished over the rang of 12.5-62.5mg/L. The concentration-effect curve seems to be bell-shaped. The data suggested that TGP hap two-sided regulatory effects on IL 2 production by splenocytes.
RESUMEN
Method for measuring interleukin 1 ( IL -1 ) and effects of total glucosides of paeony(TGP) on IL -1 production were studied. The results showed that the best concentration for LPS -induced IL-1 production of M s in rats was 8mg/L, the best dilution for IL-1 of LPS-induced M s production was 1 : 90. TGP ( 0 .5 - 12 .5mg/L ) enhanced LPS-induced IL -1 production in a concentration dependent manner, but diminished in the higher concentration of TGP ( 62 .5~l25mg/L ) , the concentration-effect curve seems to be bell-shaped. The data suggested that TGP had two-sided regulatory effects on LPS -induced IL production of M s in rats.
RESUMEN
Influences of the concentrations of luminol, zymosan & cells and opsonizing time of zymosan on luminol-dependent chemiluminescence( LDCL ) of peritoneal macrophages ( PM?) in rats were investigated by the method of orthogonal analysis. The result showed that the best levels were 100?mol/L of luminol, 10 mg/ml of zymosan, 5? 10~6/ml of cells and 45 min for opsonizing time. The LDCL of PM? was enhanced by incubation with TGP for 12 h in a concentration-dependent manner in the range of 0.09-2.25 ?g/ml of TGP, but LDCL was diminished with the concentration of 11.25 ?g/ml of TGP . The concentration-effect curve seems to be bell-shaped. The data suggested that TGP had two-sided regulatory effects on the LDCL of PM? in rats, depending on the concentration used.