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Objective To understand the HIV infection status and epidemic characteristics among blood donors in Hefei area to provide the basis for recruiting the blood donors and establishing the blood detection scheme.Methods Two kinds of serological re-agent were adopted for screening the blood donors.HRV RNA in the detection samples of the nucleic acid test system and positive serological samples were simultaneously sent to the municipal Center for Disease Control for conducting the confirmation detection from 2012.Results 495 279 blood donors were performed the serological screening,44 cases were confirmed HIV positive with the detection positive rate of 0.9;112 940 samples were performed the NAT detection,20 cases were confirmed HIV positive,all were the serological dual reagent positive samples.Conclusion The HIV infection among unpaid blood donors in Hefei area shows the increasing trend year by year.Effective measures must be taken to ensure the safety of blood.
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OBJECTIVE:To improve the HPLC-fluorometric method in the determination of telmisartan in human plasma.METHODS:Using naproxen as internal standard,the plasma was extracted with ethyl acetate.The separation was performed on Symmetry C8 with mobile phase consisted of acetonitrile -0.01mol?L-1 potassium dihydrogen phosphate buffer solution(47∶53) at a flow rate of 1.0ml?min-1.The ?Ex was set at 305nm and the ?Em was set at 365nm,and the sample size was 20?L.RESULTS:The linear range of telmisartan was 2.5～200ng?mL-1(r=0.999 8),with the lowest detectable limit at 1ng?mL-1.The average recovery of extraction was (89.91?10.07)%.Both the intra-day RSD and inter-day RSD were less than 10%.The sample showed a good stability within 24 hours after three freeze-thaw cycles and extractions.CONCLUSI-ON:The method is simple,sensitive,accurate and reproducible,and suitable for the determination and pharmacokinetic study of telmisartan in human plasma.