In vitro alpha-amylase inhibition and antioxidant activities of methanolic extract of amaranthus caudatus Linn

The present study was aimed to investigate the alpha-amylase inhibition and antioxidant activities of methanolic extract of Amaranthus caudatus Linn [MeAc]. Methanolic extract of Amaranthus caudatus was screened for alpha-amylase inhibition activity by CNPG3 method [2-chloro-p-nitrophenyl-alpha-D-maltotrioside] and antioxidant activity was evaluated by 1,1-diphenyl-2-picryl-hydrazile [DPPH] free radical scavenging, superoxide dismutase [SOD] scavenging, hydroxyl free radical scavenging, nitric oxide [NO] radical scavenging, and 2.2'-azinobis-3-ethylbenzothiazole-6-sulfonic acid [ABTS] radical scavenging assays. MeAc was also screened for non enzymatic hemoglycosylation. The methanolic extract of Amaranthus caudatus showed potent alpha-amylase inhibition activity [IC50 19.233 micro g/ml]. MeAc showed significant antioxidant activity in all the in vitro antioxidant models. Furthermore, the MeAc was found to be extremely effective in scavenging ABTS radical activity [IC50 48.75 +/- 1.1 micro g/ ml] when compared to DPPH [IC50 77.5 +/- 0.4 micro g/ml], SOD [IC50 62.5 +/- 2.1 micro g/ml], hydroxyl [IC50 88.50 +/- 1.8 micro g/ml] and NO [IC50 67.5 +/- 2.2 micro g/ml] scavenging activity. The methanolic extract of A. caudatus showed potent alpha-amylase inhibition and antioxidant activities

Estimation of gallic acid, rutin and quercetin in terminalia chebula by HPTLC

An HPTLC method was developed for the estimation of gallic acid rutin and quercetin from aqueous extrat of Terminalia chebula, precoated silica gel GF[254] as stationary phase and mobile phase for tannins toluene: acetone: glacial acetic acid [3:1:2 v/v/v/v/v] and mobile phase for rutin and quercetin, ethyl acetat: dichloromethane: formic acid: glacial acetic acid: water [10:2:5:1:1:0.1, v/v/v/v/v]. Detection and quantification were performed densitometrically at lambda = 254 for gallic acid and 366 nm for rutin and quercetin. The R[f] values of gallic acid, rutin and quercetin] are 0.30, 0.13 and 0.93 respectively. The total peak areas of the standards [gallic acid, rutin and quercetin] and the corresponding peak areas of extracts were compared and the Gallic acid, rutin and quercetin content was estimated to be 8.380, 0.170 ad 0.10331% w/w