RESUMEN
Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes
Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared
Methods: Glucose uptake measurement was performed using 2-[N-[7-nitrobenzin-2-oxa-1, 3- diazol-4-yl] amino]-2-deoxyglucose [2-NBDG] and Omega FluoStar plate reader. Incubation of cells [10[4]/ml, in 24 well plate at 37 [degree sign]C in 5% CO[2] / air] with three phytoestrogens at concentration of 10[-4] M to 10[-9]M in two studies mood, short term treatment [one hour] and long term treatment [24 hours] was tested
Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 micro M, which stimulated uptake about three-fold compared with control. Longer treatments with 10[-4]M to 10[-6] M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations
Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA