RESUMEN
OBJECTIVE: To study the optimum extraction condition of triterpene acids in ldregs of leaves of Eriobotrya japonica (Thunb.) Lindl. METHODS: Single factor analysis and orthogonal experimental design were used to select the concentration of ethanol (A), solid-liquid ratio (B), extraction time (C), and extraction times (D) by L9(34) orthogonal test. RESULTS: The concentration of ethanol was the main process factor, and extraction times was the secondary factor. The optimum condition was A2B1C3D1, which included using 90% ethanol as the extraction solvent with 7 times the amount of φ, and 80°C heat back for 2 times, 1.5 h for each time. CONCLUSION: Ethanol has good extraction effect for triterpene acids in loquat residue. This study provides important information for the comprehensive utilization of leaves of Eriobotrya japonica (Thunb.) Lindl. resources by pharmaceutical manufacturers.