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@#Objective To explore the restoration of motor function and the expression of calcitonin gene-related peptide(CGRP)and acetylcholine esterase(AChE)in the anterior horn motoneurons after different types of spinal cord injury.Methods 60 adult female Wistar rats were randomly assigned to 3 groups:sham group,completely transection group and contusion group.Average combined scores(ACOS)were applied to assess the motor function at various time after the surgery.The content of AChE in the anterior horn of L2-L4 was detected with Karnovsky-Roots staining and the expression of CGRP was then determined with immunohistochemistry.Results The scores of ACOS were much higher in the contusion group than in the transection group at each time point examined.The content of both AChE and CGRP significantly decreased after either type of spinal cord injury.However,their activity gradually recovered to the normal level in the contusion group,but not in the transection group.Moreover,the changes of CGRP occurred earlier than those of AChE.Conclusion There is strong relationship between the motor function recovery and the functional state of anterior horn cells.CGRP or AChE may play an important role in the functional recovery of locomotion after spinal cord injury in rats.
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@#ObjectiveTo study the effect the chitosan tube combined with the bio active carrier system on inducing nerve axon regeneration of rats with spinal cord injury.Methods50 female Wister rats were characterized by the right spinal cord hemisections at the seventh and eighth thoracic segment to make the model of spinal cord hemisection. The chitosan tube serving as a regenerative loculus was implanted in the defect location of the experimental models.In the experimental group,the bio active carrier system was injected into the chitosan tube,while in control group injected nothing.The drua was sutured to restore cerebrospinal fluid circulation.Results6 months and 12 months after operation,the regenerative nerve axon had passed the defect area of spinal cord in the experiment group. According to WGA-HRP anterograde axonal tracing study, some TMB-positive axons were observed in the distal graft host interface,and came into the host environment. TEM-ultrastructure indicate some neonate synapse, myelinated nerve fibers.In the control group,few regenerative axon could be seen, there was no regenerative axon pass the middle of the tube.ConclusionsThe chitosan tube in which the bio active carrier system was injected can induce the spinal cord nerve axon regeneration.