Expression and clinical significance of long chain non-coding RNA LOC285194 in human breast cancer tissue / 重庆医学

Objective To investigate the expression of long chain non-coding(lnc) RNA LOC285194 in breast cancer tissue and paracancerous tissue and its clinical significance.Methods Forty-two samples of paraffin embedded breast cancer tissue and 16 samples of paraffin embedded paracancerous tissue were selected.The expression of lncRNA LOC285194 in these tissue were detected by using quantitative real-time polymerase chain reaction(PCR).Then its correlation with clinicopathological features was analyzed.Results The expression level of lncRNA LOC285194 in breast cancer tissue was significantly lower that in the paracancerous tissue (P＜0.01);the level of lncRNA LOC285194 in human epidermal growth factor receptor-2(HER2)overexpression tissues was up-regulated compared with HER2 negative breast cancer tissue(P =0.013),there was a positive correlation between them(r=0.385,P=0.012).Conclusion lncRNA LOC285194 may play the role of cancer suppressor gene and may be involved in the generation of breast cancer by HER2 association,which may become a target gene of breast cancer treatment.

Research Progress on the relationship between HPV E6 protein and cervical cancer / 医学研究生学报

Cervical cancer is the second most common malignant tumor of women all over the world.Infection with oncogenic human papilloma virus ( HPV) types is the most important risk factor to cause cervical cancer.The viral genome of HPV consists of three regions:the early region (E), the late region (L) and the long control region (LCR).The early proteins (E1, E2, E4, E5, E6, E7 ) play vital roles in the continuous virus expression and replication in cells.Among these proteins, E6 protein has the closest relationship with the tumorogenesis and development of cervical cancer.Many mechanisms of the HPV encoded early protein E6 in in-ducing cervical cancer have been characterized, including degradation of p53 protein, activation of telomerase, interaction with PDZ protein, disturbance of cellular signaling pathways, inhibition of immune recognition and blockage of cellular apoptosis.HPV E6 pro-tein may be a preferred target in prevention and treatment of cervical cancer.