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1.
Indian J Exp Biol ; 2005 Jun; 43(6): 561-5
Artículo en Inglés | IMSEAR | ID: sea-56095

RESUMEN

In vitro clonal multiplication of apple rootstock MM 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5 - 1.0 mgl(-1)), GA3(0.5 mgl(-1)), with or without IBA(0.05 - 0.1 mgl(-1)). Small size explants showed less phenol exudation and less contamination. Following establishment phase, the small shoots emerged from explants were subcultured on MS medium supplemented with different combinations and concentrations of growth regulators. BA (1.0 mgl(-1)) and GA3 (0.5 mgl(-1)) combination showed highest multiplication rate (1:5), andcl also produced longer shoots. Two step rooting was done by transferring microcuttings to auxin free solid medium after root initiation in dark on 1/2 strength MS liquid medium containing IBA (0.5 mgl(-1) ). Rooted plantlets were transferred to peat containing paper cups and resulting plants of MM 111 acclimated successfully for transfer to field.


Asunto(s)
Técnicas de Cultivo de Célula/métodos , Clonación Molecular/métodos , Medios de Cultivo/farmacología , Técnicas de Cultivo , Frutas , Ácidos Indolacéticos , Malus/citología , Reguladores del Crecimiento de las Plantas/metabolismo , Hojas de la Planta/metabolismo , Raíces de Plantas/metabolismo , Brotes de la Planta/metabolismo
2.
Indian J Physiol Pharmacol ; 1996 Oct; 40(4): 309-17
Artículo en Inglés | IMSEAR | ID: sea-107264

RESUMEN

Thirteen healthy subjects were tested for parasympathetic reactivity during head-up tilt and reversal of the tilt. Head-up tilt (70 degrees) resulted in significant increase in baseline heart rate and diastolic blood pressure. Head-up tilt also led to increased parasympathetic reactivity as measured by Valsalva manoeuvre and hand grip test. Heart rate response to deep breathing test did not change. The reversal of the tilt led to returning of heart responses to original values. Responses indicate towards enhanced parasympathetic reactivity during head-up tilt position.


Asunto(s)
Adulto , Presión Sanguínea/fisiología , Ejercicio Físico/fisiología , Frecuencia Cardíaca/fisiología , Humanos , Sistema Nervioso Parasimpático/fisiología , Postura/fisiología , Respiración/fisiología , Pruebas de Mesa Inclinada , Maniobra de Valsalva
3.
Indian J Exp Biol ; 1996 Jun; 34(6): 508-12
Artículo en Inglés | IMSEAR | ID: sea-60087

RESUMEN

The excretory-secretory (E-LS) products released by the adult Setaria cervi, a bovine filarial parasite, were used to raise polyvalent hyperimmune serum in rabbits. Analysis of E-S products, using anti-E-S serum showed the presence of 10-14 immunogenic proteins, the rabbit anti-E-S serum showed reciprocal antibody titres in the range of 100,000-250,000 by enzyme linked immunosorbent assay. The anti-E-S antibodies could detect circulating antigen in filarial patients sera by Counter immunoelectrophoresis.


Asunto(s)
Animales , Antígenos Helmínticos/sangre , Bovinos , Filariasis/diagnóstico , Humanos , Pruebas Inmunológicas/métodos , Setaria (Nematodo)/inmunología
5.
Indian J Exp Biol ; 1995 Jan; 33(1): 6-11
Artículo en Inglés | IMSEAR | ID: sea-55652

RESUMEN

Lactate dehydrogenase (LDH) of malarial parasites has been demonstrated to be biochemically and immunochemically distinct from the equivalent host enzyme. The polyclonal antibodies raised against the purified plasmodial LDH showed specificity to Plasmodium spp. Six hybridoma cell lines secreting monoclonal antibodies specific to Plasmodium knowlesi LDH have been obtained. The two monoclonal antibodies (2A3B7 and 4A6A7) showed high reactivity with LDH from simian (P. knowlesi. P. cynomolgi), human (P. falciparum, P. vivax) and rodent (P. berghei, P. yoelii) malarial parasites and did not cross-react with red cell LDH as well as with isoenzymic forms of mammalian LDH (A4, B4 and C4). One monoclonal antibody (4A6A7) strongly inhibited the enzyme activity specifically of plasmodial LDH and did not have any effect on the activity of red cell LDH. The other monoclonal (2A3B7) did not show inhibitory effect on parasite LDH. These findings as well as competitive immunoassay studies suggest the presence of at least two parasite specific epitopes on plasmodial LDH.


Asunto(s)
Animales , Anticuerpos Monoclonales , Especificidad de Anticuerpos , Ensayo de Inmunoadsorción Enzimática , Humanos , L-Lactato Deshidrogenasa/inmunología , Plasmodium knowlesi/inmunología
6.
Indian J Exp Biol ; 1994 Jun; 32(6): 371-5
Artículo en Inglés | IMSEAR | ID: sea-59012

RESUMEN

Several common antigens between the bovine (Setaria cervi) and human (Brugia malayi) filarial parasites have been demonstrated [Immunol Investig, 16 (1987) 139]. Hybridoma cell lines producing monoclonal antibodies against such common antigenic epitopes were obtained by immunizing the BALB/c mice with S. cervi antigen, fusing the spleen cells with Sp2/0 myeloma cells and screening the culture supernatants for antibody against both S. cervi and B. malayi antigens by ELISA. Nine monoclonal antibodies directed against antigenic epitopes common between the bovine and human filarial parasites were identified. Two monoclonal antibodies (I3B4 and I5D6) showed reactivity with the antigen(s) present in filariasis patients serum and thus may have potential for detecting circulating antigen in filaria infected individuals.


Asunto(s)
Animales , Anticuerpos Monoclonales , Antígenos Helmínticos/inmunología , Brugia Malayi/inmunología , Epítopos , Femenino , Masculino , Setaria (Nematodo)/inmunología
7.
Indian J Exp Biol ; 1990 Sep; 28(9): 871-5
Artículo en Inglés | IMSEAR | ID: sea-60249

RESUMEN

Localization of different enzymes of PEP-succinate pathway has been done in Setaria cervi, a bovine filarial worm. Succinate dehydrogenase and fumarate reductase were localized in mitochondria rich particulate fraction while all other enzymes were cytosolic. The in vitro effect of certain antifilarial/anthelmintic agents on these enzymes was also investigated. Sumarmin, at low concentration, could cause a marked inhibition of most of the enzymes of this pathway. Centperazine, an antifilarial drug being developed by CDRI showed significant inhibitory action on pyruvate kinase, lactate dehydrogenase, fumarase and succinate dehydrogenase while CDRI compound 72/70 showed significant inhibition of PEP-carboxykinase activity. Diethylcarbamazine and levamisole, however, were found to be more or less ineffective at lower concentrations against all the enzymes of this pathway.


Asunto(s)
Animales , Antihelmínticos/farmacología , Femenino , Filarioidea/enzimología , Fosfoenolpiruvato/metabolismo , Setariasis/parasitología , Succinato Deshidrogenasa/metabolismo , Succinatos/metabolismo
8.
Indian J Biochem Biophys ; 1989 Feb; 26(1): 52-5
Artículo en Inglés | IMSEAR | ID: sea-27489

RESUMEN

A comparative analysis of surface proteins of adult, microfilariae and infective larvae of Brugia malayi, the human filarial parasite, has been carried out using IODOGEN (1,3,4,6-tetrachloro-3,alpha 6 alpha-diphenyl-glycoluril) and lactoperoxidase methods. SDS-polyacrylamide gel electrophoretic and autoradiographic analyses revealed the presence of 9 proteins (15-200 kDa) in adults, while microfilariae and infective larvae showed 8 and 6 proteins (15-120 kDa), respectively. The pattern of proteins radiolabelled by IODOGEN method was very similar to that of proteins labelled by the lactoperoxidase method. Since these proteins are released by the protease treatment of whole parasites, they are likely to be present on the surface of the parasite.


Asunto(s)
Animales , Brugia/análisis , Radioisótopos de Yodo/diagnóstico , Lactoperoxidasa , Larva/análisis , Proteínas de la Membrana/análisis , Microfilarias/análisis , Urea/análogos & derivados
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