RESUMEN
Aim: We describe the minimum requirements and a simplified method for isolation and characterization of mesenchymal stem cells [MSCs] from human bone marrow
Background: MSCs are well known adult stem cells present in many tissues such as adipocytes, chondrocytes, osteoblasts, and neurons. Many isolations and characterization methods have emerged to apply MSCs in the clinical applications, which many of them are expensive and time-consuming
Methods: MSC isolation was carried out from human bone marrow, and cultured in defined medium. Cultures were maintained at 370C in a humidified atmosphere containing 5% CO2 for 48h. The medium was exchanged every 3-4 days. Adherent cells were characterized according to main criteria defined by ISCT, such as differentiation capability to adipocyte and osteoblast using specific differentiation mediums; also, flow cytometry verified MSC specific markers
Results: Isolated MSCs had a fibroblastic-like appearance with adherent property to the culture plate. Differentiation function was proved with the formation of lipid drops and calcium oxalates on the differentiated MSCs and finally, purified MSCs from bone marrow were positive for cell surface markers, CD73, CD90, and CD105 while being negative for CD34 and CD45
Conclusion: These findings confirm that the represented method is capable of isolating MSCs from bone marrow with proven results according to all minimum criteria defined by the International Society for Cellular Therapy [ISCT]
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Aim: This study investigated subtypes of Cryptosporidium in patients with gastrointestinal complaints in Tehran, Iran
Background: Cryptosporidium, an intracellular protozean parasite, is among the major causative agents of gastroenteritisdisorders in humans. It also causes water-borne and food-borne outbreaks of diarrheal diseases
Patients and methods: A total of 1685 fecal samples were collected from patients with gastrointestinal complaints whohad been referred to clinical laboratories Tehran, Iran. The primary diagnosis was established by the detection of oocystsusing the modified Ziehl-Neelsen staining method and following that, the positive microscopically samples wereselected for sequence analysis of the partial 60 kDa glycoprotein [gp60] gene
Results: Out of 1685 collected samples, 7 [0.4 %] were positive for Cryptosporidium oocysts. Sequence analysis of gp60gene in seven Cryptosporidium isolates revealed that two subtype families were identified, IIa and IId. Five [of 7] isolatesbelonged to the subtype family IIa and the remaining two isolates belonged to IId. Two sub-types were recognized within thesubtype family II,a including IIaA16G2R1 [3/5], IIaA17G1R1 [2/5], while IIdA17G1d was the only subtype within IIdsubtype family
Conclusion: The predominance of zoonotic subtype families of C. parvum species [IIa, IId] in this study highlights theimportance of zoonotic transmission of cryptosporidiosis in the country
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Helicobacter pylori is an important pathogen for gastroduodenal diseases. Infection with H. pylori can be limited by regimens of multiple antimicrobial agents. However, antibiotic resistance is a leading cause of treatment failure. The aim of this study has been to determine the resistance patterns of H. pylori strains isolated from gastric biopsies of patients with dyspepsia by agar dilution method, in Tehran, Iran. H. pylori isolates from patients with gastrointestinal diseases were evaluated for susceptibility testing by agar dilution method. Susceptibility testing was performed to commonly used antibiotics including clarithromycin, tetracycline, amoxicillin, metronidazole and ciprofloxacin. Among 92 patients with dyspepsia, H. pylori strains were isolated from 42 patients. Seventeen [40.5%] of the isolates were resistant to metronidazole [MICs >/= 8 microg/1], whereas one isolate [2.4%] was resistant to amoxicillin [MICs