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Chinese Journal of Virology ; (6): 466-470, 2007.
Artículo en Chino | WPRIM | ID: wpr-334864

RESUMEN

This paper was to study the angiogenic inhibitory effect and the potential antitumor effect of the constructed recombinant DNA vaccine CRT/HPV6bE7 in vivo. The C57BL/6 mice were vaccinated respectively with recombinant CRT/HPV6bE7 DNA plamids. The inhibitory effects on angiogenesis of generated vaccines in vivo were evaluated by a bFGF-induced angiogenesis assay using the Matrigel kit. To investigate the potential antitumor effect, the mean tumor weights, sizes and tumor appearing times were measured in C57BL/6 mice treated with HPV6bE7-expressing B16 cells. The results indicated that the recombinants CRT180/HPV6bE7 and CRT180 showed strong anti-angiogenic effects in bFGF-induced angiogenesis in vivo. Moreover, CRT180/HPV6bE7 and CRT180 DNA vaccines could significantly inhibit the tumor growth in tumor challenge experiment, and CRT180/HPV6bE7 was superior to other vaccines in delaying tumor formation time, limiting tumor size and weight in tumor protection experiment. In conclusion, recombinant CRT180/HPV6bE7 DNA could elicit a most efficient anti-angiogenic effect and inhibit tumor growth in mice inoculated with DNA vaccines. The antiangiogenic activity of CRT were suggested residing in a domain between CRT 120-180 aa.


Asunto(s)
Animales , Femenino , Ratones , Calreticulina , Genética , Papillomavirus Humano 6 , Alergia e Inmunología , Ratones Endogámicos C57BL , Neovascularización Patológica , Proteínas Oncogénicas Virales , Genética , Infecciones por Papillomavirus , Terapéutica , Vacunas contra Papillomavirus , Alergia e Inmunología , Proteínas Recombinantes de Fusión , Genética , Vacunas de ADN , Alergia e Inmunología
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