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1.
Artículo en Inglés | IMSEAR | ID: sea-16197

RESUMEN

The effect of sperm specific lactate dehydrogenase-C4 (LDH-C4) alone or with muramyl dipeptide (MDP) and Freund's complete adjuvant (FCA) on immune responses and breeding capacity have been studied in isogeneic C57 BI/Ks (H-2d) mice. Results per se suggested that LDH-C4 generates isoantibodies even in absence of adjuvant. Though MDP could be substituted for FCA as adjuvant, amplification by MDP of the antibody levels is reduced to half that obtained with FCA. LDH-B4 from kidney did not produce any antibody response under similar conditions. Systemic immunization with LDH-C4 did not reduce the overall pregnancy rate but reduced the frequency of embryo resorption and increased litter size significantly. However, mothers with foetal non-resorption had in general, lower antibody titres than the mothers showing foetal resorptions. The popliteal lymph node (PLN) assay for local graft versus host (LGVH) reaction revealed that the maternal lymphocyte cell competence was depressed by the sperm specific isozyme during gestation; depression in stimulation index was associated with the low antibody titre group. MDP, like LDH-C4 alone did not modify LGVH reaction significantly although it exerted a similar effect as LDH-C4 in embryo protection.


Asunto(s)
Acetilmuramil-Alanil-Isoglutamina/administración & dosificación , Animales , Formación de Anticuerpos , Femenino , Fertilidad/inmunología , Adyuvante de Freund/administración & dosificación , Inmunización , Isoenzimas , L-Lactato Deshidrogenasa/administración & dosificación , Masculino , Ratones , Ratones Endogámicos C57BL , Distribución Aleatoria , Espermatozoides/enzimología
2.
Indian J Biochem Biophys ; 1992 Jun; 29(3): 271-6
Artículo en Inglés | IMSEAR | ID: sea-26691

RESUMEN

Immune responses to a well-defined sperm-specific isogenic lactate dehydrogenase-C4 (LDH-C4) have been studied in C57Bl/Ks (H-2d) mice after immunization through intra-rectal route. Presence of anti-LDH-C4-antibodies in the sera of females immunized in presence or absence of adjuvant suggested that the immune system of mice becomes exposed to sperm antigens following intrarectal insemination. LDH-C4 primed lymphocytes from both males and females, when transferred in F1 hybrids, suppressed stimulation index of local graft versus host reaction. However, contrary to females, male counterparts which did not elicit measurable anti-LDH-C4-antibody titer, showed the presence of a higher proportion of Ly2+ and Ia+ fluorescence labelled cells in the spleen of LDH-C4 administered mice. Results suggest that males are more susceptible for immune suppression of T cell functions through generation of T suppressor cells. Sex differences in relation to immune deviation by intra-rectal administration of sperm-specific LDH-C4 in mice and their consequences in AIDS and AIDS-related complex diseases are described.


Asunto(s)
Animales , Anticuerpos Monoclonales , Formación de Anticuerpos/efectos de los fármacos , Femenino , Citometría de Flujo , Reacción Injerto-Huésped , Isoenzimas , L-Lactato Deshidrogenasa/aislamiento & purificación , Masculino , Ratones , Ratones Endogámicos , Bazo/inmunología , Linfocitos T/efectos de los fármacos , Testículo/enzimología
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