RESUMEN
Three hundred and seventy-six consecutive antinuclear antibody-positive sera were tested for anti-ds DNA antibody by using three commercial kits which use 125 I recombinant DNA [radioimmunoassay], highly purified calf thymus DNA [enzyme linked immunosorbent assay] and Crithidia lucilliae [immunofluorescence assay] as substrates. All patients' sera, after reviewing medical records, were classified into three broad groups: Group I [systemic lupus erythematosus], Group II [rheumatic diseases and rheumatoid arthritis], and Group III [nonspecific ANA antibody test positive]. A sensitivity, specificity, positive predictive test value and negative predictive test value for Group I against Group II-III [generally these two groups of sera should not show any anti-ds DNA antibody] combined showed for Crithidia lucilliae [IF assay] 58.8%, 93.6%, 82% and 82%, for 125 I recombinant DNA [RIA] assay, 75.8%, 94%, 86.2% and 88.7% and calf thymus highly purified DNA [ELISA] assay using positive cut-off value >100 U/mL, 97.5%, 35%, 42.9% and 24%. The 125 I recombinant DNA [RIA] assay based on the principle of the Farr technique, which is still considered to be the gold standard for anti-ds DNA antibody detection, showed the best specificity and sensitivity among all three methods tested in this study
Asunto(s)
Juego de Reactivos para Diagnóstico/normas , ADN Recombinante , Radioinmunoensayo/métodos , /métodos , Técnicas de Inmunoadsorción/métodosRESUMEN
Anti-T-lymphotrophic virus type 1 [HTLV] antibody concentration was determined by enzyme-linked immunosorbent assay in 153 sera specimens from patients with histologically proved non-Hodgkin's lymphoma. The presence of anti-HTLV-1 virus antibody was demonstrated in 5.2% [8/153]
RESUMEN
Anti-delta-virus antibody was determined by readioimmunoassay technique in 490 healthy male Saudi blood donors with serum positive for hepatitis B surface antigen. The presence of anti-delta-virus antibody was demonstrated in 11.2% [55/490] of donor sera. This is considerably higher than the reported incidence of 3.8%, 0.5%, and from the United states, Canada, and 8%, 6.7%, and 5.4% from Saudi Arabia in healthy HB[s]Ag-positive blood donor sera
Asunto(s)
Donantes de Sangre , Hepatitis DRESUMEN
Hepatitis B surface antigen was determined by radiommunoassay in 2487 male Saudi and 7587 Western expatriate volunteer blood donors. The HB[s]Ag positivity rate was 8.5% in male Saudi and 0.7% in Western expatriale blood donors. This study was further extended to test other hepatitis B virus [HBV] markers and alanine aminotransferase [ALT] in 200 consecutive Saudi male blood donors' sera. A positivity rate for HB[s]Ag was 8.5%, anti-Hbs wasas 35.5%, anti-HB[c] [alone] was 1.5%, HB[e]Ag was 1.0%, and anti-HBe was 2.5%. A suggested nonspecific screening test for ALT enzyme with levels of >/= 45 IU/L [normal 6-36 IU/L] for non-A, non-B hepatitis carrier state was performed on the 200 male Saudi blood donor sera, using Dupont ACA III methodology. The result showed an elrvation of enzyme in 2.5% of Saudi donors. If a blood donor with any HBV marker were excluded then only 1.5% showed ALT enzyme elevation, considerably lower than the reported 3.6% in the U.S. blood donor population. The positivity rates of 8.5% for HB[s]Ag, 35.5% for anti-HB[s] and 49% for any HBV serological markers in male Saudi blood donors are significantly higher than in American volunteer blood donors
Asunto(s)
Donantes de SangreRESUMEN
The present study was undertaken to determine the prevalence rate of hepatitis B in the patient population and the risk of contracting hepatitis B by health care workers in this comprehensive care hospital. The sera of 500 consecutive admissions were tested hepatitis B serological markers using sensitive radioimmunoassay methods. The significant positive results show: hepatitis B 'surface' antigen [HB[s]Ag], 8.8%; antiHB[c] ['core' alone], 3.4%; and, anti-HB[s] 46.6%. There was at last one positive hepatitis B virus marker in 56.6% of those tested. The presence of HB'eAg, suggesting high infectivity, was detected in 9% of HBsAg positive sera. An 8.8% HB[s]Ag and 3.4% anti-HB[c] [alone] diction rate gives a total of 12.2% of patients who potentially may transmit hepatitis B to health care workers at this hospital. This is significantly higher than the 0.3 to 2% reported from U.S general metropolitan hospital. The presence of a hepatitis B markers inn 56.6% of the patient population places health care workers of this hospital at considerable risk of contracting hepatitis B, compared to that in North American hospital