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Asunción; EFACIM; may; 1994. 26-31 p. ilus, tab.
Monografía en Español | LILACS, BDNPAR | ID: biblio-1017958

RESUMEN

In an attempt to clone some specific parasite antigen genes, a Trypanosoma cruzi cDNA library was constructed in lambda gt11. Efficient isolation of undegraded mRNA from total RNA was obtained which is a critical step for the construction of representative cDNA libraries. Lambda gtll recombinant phages were detected using isopropylthiogalactoside (IPTG) and 5-bromo-4-chloro-3-indoly1-b-d-galactopiranoside (X-gal) detection system


Asunto(s)
ADN , Trypanosoma cruzi
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