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1.
Acta cir. bras ; 35(4): e202000405, 2020. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1130632

RESUMEN

Abstract Purpose To investigate the effects of Chemically Extracted Acellular Nerves (CEANs) when combined with Adipose-Derived mesenchymal Stem Cell (ADSC) transplantation on the repair of sciatic nerve defects in rabbits. Methods A total of 71 six-month-old Japanese rabbit were used in this study. Twenty rabbits served as sciatic nerve donors, while the other 51 rabbits were randomly divided into Autologous Nerve Transplantation Group (ANT, n=17), CEAN group (n=17) and CEAN-ADSCs group (n=17). In all these groups, the rabbit's left sciatic nerves were injured before the experiment, and the uninjured sciatic nerves on their right side were used as the control (CON). Electrophysiological tests were carried out and sciatic nerves were prepared for histomorphology and stretch testing at 24 weeks post-transplant. Results There were significant differences between ANT and Con groups in amplitude (AMP): P=0.031; motor nerve conduction velocity (MNCV): P=0.029; Maximum stress: P=0.029; and Maximum strain P=0.027. There were also differences between the CEAN and CEAN+ADSCs groups in AMP: P=0.026, MNCV: P=0.024; Maximum stress: P=0.025 and Maximum strain: P=0.030. No significant differences in these parameters were observed when comparing the ANT and CEAN+SACN groups (MNCV: P=0.071) or the CEAN and ANT groups (Maximum stress: P=0.069; Maximum strain P=0.077). Conclusion Addition of ADSCs has a significant impact on the recovery of nerve function, morphology, and tensile mechanical properties following sciatic nerve injury.


Asunto(s)
Animales , Masculino , Neuropatía Ciática/cirugía , Neuropatía Ciática/fisiopatología , Trasplante de Células Madre Mesenquimatosas/métodos , Células Madre Mesenquimatosas , Tejido Nervioso/trasplante , Conejos , Valores de Referencia , Nervio Ciático/cirugía , Nervio Ciático/fisiopatología , Fenómenos Biomecánicos , Reproducibilidad de los Resultados , Resultado del Tratamiento , Electromiografía , Regeneración Nerviosa/fisiología , Tejido Nervioso/cirugía
2.
Acta cir. bras ; 33(10): 935-944, Oct. 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-973465

RESUMEN

Abstract Purpose: To investigate the impact of bone mesenchymal stem cells (BMSCs) intervention on the viscoelasticity of sciatic nerve in rats with chronic alcohol intoxication (CAI). Methods: The CAI rat models were prepared, divided into model groups, and treated with either BMSCs or basic fibroblast growth factor (bFGF). Then the rats underwent electrophysiological test and the serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), and metallothionein (MT) were measured. Histological observation, stress relaxation test, and creep test were performed for the sciatic nerve of the CAI model in each group. Results: The MDA level of group BMSC was significantly lower (p<0.05) than that of groups MOD (the CIA model) and bFGF. The SOD and MT levels were higher in group BMSC than in groups MOD and bFGF (p<0.05). The motor nerve conduction velocity and amplitude were higher in group BMSC than in groups MOD and bFGF (p<0.05). The amounts of 7200s stress reduction and 7200 s strain increase of the sciatic nerve in group BMSC were greater than those in groups bFGF and MOD (p<0.05). Conclusion: Bone mesenchymal stem cells can improve the metabolism of free radicals, restore the tissue morphology and viscoelasticity of the chronic alcohol intoxication animal model, and positively affect the repairing of the injured sciatic nerve.


Asunto(s)
Animales , Masculino , Ratas , Nervio Ciático/fisiopatología , Trasplante de Médula Ósea , Trasplante de Células Madre Mesenquimatosas , Intoxicación Alcohólica/fisiopatología , Regeneración Nerviosa , Nervio Ciático/patología , Estrés Fisiológico , Superóxido Dismutasa/sangre , Viscosidad , Células de la Médula Ósea , Factor 2 de Crecimiento de Fibroblastos , Ratas Wistar , Modelos Animales de Enfermedad , Intoxicación Alcohólica/sangre , Elasticidad , Malondialdehído/sangre , Metalotioneína/sangre
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