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PURPOSE: Human umbilical cord blood as a potential source of hematopoietic stem cell for stem cell transplantation in children has recently been advocated. Clinical application of cord blood transplantation, however, requires adequate blood volume and number of stem cells. Currently, the number of stem cells in the cord blood is usually measured by flowcytometry, which requires strict quality control and high costs. Here, we postulate that the number of nucleated red blood cells(NRBC) which are relatively immature erythroid cells may correlate well with that of CD34-expressing(CD34+) cells which are considered lymphohematopoietic precursors. If so, CD34+ cell-rich cord blood can be selected and such cells enumerated by a simple and cost-effective blood smears. METHODS: Correlation between CD34+ cell and nucleated RBC in human cord blood were checked. Sixty cord blood specimens(30 specimens for group A with NRBC>500/mul and 30 specimens for group B with NRBC500/ul; 55+/-61(SD))(p<0.005). 3.Regression relationship among CD34+ cell, WBC, and NRBC counts was as following. ln(CD34+ cell) = -12.21 + 1.46xln(WBC)+ 0.25xln(NRBC) gamma2=30.07 p=0.0001 4.There was a significant correlation between CD34+ cell counts and NRBC counts(gamma=0.4334, p<0.005) CONCLUSIONS: Our results suggest that there is a significant correlation between CD34+ cell counts and NRBC counts, and that CD34+ cell-rich cord blood specimens can be selected by a simple NRBC counting.
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Niño , Humanos , Recién Nacido , Volumen Sanguíneo , Recuento de Células , Trasplante de Células Madre de Sangre del Cordón Umbilical , Ácido Edético , Células Eritroides , Sangre Fetal , Células Madre Hematopoyéticas , Microscopía , Control de Calidad , Trasplante de Células Madre , Células MadreRESUMEN
No abstract available.
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The authors report a case of chylous ascites in a two month old boy with massive ascites. He was managed with repeated therapeutic paracentesis and skimmed milk feeding. A marked improvement was resulted in clinical symptoms and laboratory findings at the time of discharge to be followed-up at the outpatient service. A brief review of literatures is included
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Humanos , Lactante , Masculino , Ascitis , Ascitis Quilosa , Leche , Pacientes Ambulatorios , ParacentesisRESUMEN
Histopathological findings, clinical picture, various laboratory data and correlation of them to response of the patients to steroid therapy were analyzed in 9 nephrotic who had been admitted to the Seoul National University Hospital during the period of 3 years and 6 months from March of 1975 till September of 1978, and who were confirmed to have pure mesangial proliferative glomerulonephritis by renal histology. All patients were followed-up at least 1 year, up to 3 years. Age of the patients at the onset was widely distributed from 2 to 14 years, but most patients, (7 cases in 9) were after the age of 6 years. Selected findings of blood and urine examinations revealed typical patterns of the nephrotic syndrome and there was no case without edema of any degree. Histopathological study showed characteristic glomerular mesangial hypercellularity and lobular stalk widening. According to severity of the glomerular mesangial hypercellularity the patients were divided into 2 groups, one with milder changes and the other with more severe ones. There were no difference of degree of symptoms, laboratory data and response to steroid therapy between the 2 groups of the patients. But the pattern of response to steroid therapy as a whole was conspicuously different from that seen in cases with minimal change lesion. During the observation period, there were 2 cases of relapse, but case of renal failure or death was, absent possibly because the observation period was short. There was no case who developed serious side effect of steroid so as to stop administration of the drug.