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1.
J. appl. oral sci ; 24(4): 338-343, July-Aug. 2016. tab, graf
Artículo en Inglés | LILACS, BBO | ID: lil-792602

RESUMEN

ABSTRACT The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.


Asunto(s)
Animales , Ratones , Factor de Necrosis Tumoral alfa/análisis , Resinas Compuestas/toxicidad , Resinas de Silorano/toxicidad , Metacrilatos/toxicidad , Valores de Referencia , Factores de Tiempo , Ensayo de Materiales , Ensayo de Inmunoadsorción Enzimática , Línea Celular , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Células Cultivadas , Reproducibilidad de los Resultados , Resinas Compuestas/efectos de la radiación , Luces de Curación Dental , Resinas de Silorano/efectos de la radiación , L-Lactato Deshidrogenasa , Metacrilatos/efectos de la radiación
2.
RSBO (Impr.) ; 12(3): 266-271, Jul.-Sep. 2015. ilus, tab
Artículo en Inglés | LILACS | ID: biblio-842376

RESUMEN

Introduction and objective:The purpose of the study was to evaluate different pH cycling protocols on the induction of artificial carious lesions in bovine dentin, since the most appropriate protocol to be applied is still not fully established. Material and methods: Fragments of bovine dentin (4 x 4 x 2 mm) were embedded in resin, polished and 7 mm² of each fragment was isolated with wax. The specimens were divided into three groups (A, B, C) according to the time of immersion in the demineralizing solution (1.5 ml). Group A - 15 minutes; Group B - 30 minutes; Group C - 60 minutes and subsequently immersed for 22 hours in a remineralizing solution (1.5 ml). Microhardness measurements were conducted initially, daily and after each pH cycling for 4 days. The Split-plot design (ANOVA) was applied. Results: There was a significant interaction between time and cariogenic challenge (p<0.0001). Bonferroni comparisons were executed to identify the differences over the cariogenic challenge, showing that increasing the immersion time in demineralizing solution for each pH cycling assessed, the cariogenic challenge aggressiveness increased (A

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