Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
Artículo en Inglés | IMSEAR | ID: sea-162901

RESUMEN

Aim: Considering the geographic expansion of Cryptococcus gattii, the aim of this study was to investigate hollows of living trees as a reservoir of C. gattii in Rio de Janeiro, Brazil. Place and Duration of the Study: In an urban quarter of Rio de Janeiro city, 80 samples of decaying wood were collected. In addition, 85 decaying wood samples were collected in the wild rainforest. The samples were analyzed at the Mycology Laboratory, Evandro Chagas Clinical Research Institute, Oswaldo Cruz Foundation, from 2008-2010. Methodology: Samples were collected by scraping the inner decaying wood of the hollows of the trunks of each tree. Pathogenic Cryptococcus species were identified by: brown colonies on niger seed agar (NSA) medium, thermotolerance at 35ºC, cycloheximide sensitivity, carbon and nitrogen assimilation tests performed by 32-Vitek System (Vitek ICB, bioMeriux, Durham, EUA). Canavanine-glycine-bromothymol blue medium (CGB) was used to determine the species of the isolates and the genotypes were determined by restriction fragment length polymorphism of URA5 gene. Results: After plating the samples on NSA, 584 colonies were obtained from the urban quarter. C. gattii VGI was identified in 98% of colonies, followed by C. neoformans VNI 2%. The positivity of the urban area was 7.8%. The concentrations of the fungi in hollows of ficus trees ranged from 50 to 56,250 colony-forming units per gram of sample (CFU/g). Conclusions: For the first time in Rio de Janeiro C. gattii VGI was isolated in a hollow of living tree.

2.
Mem. Inst. Oswaldo Cruz ; 104(4): 662-664, July 2009. ilus
Artículo en Inglés | LILACS | ID: lil-523738

RESUMEN

Cryptococcus neoformans and Cryptococcus gattii are important agents of meningoencephalitis in humans in the city of Belém. This clinical data suggests that the region may be a highly endemic area for the pathogenic Cryptococcus species within the state of Pará (PA), Northern Brazil. Preliminary analysis of 11 environmental samples from the city of Belém showed two positive locations, including a hollow of a kassod tree (Senna siamea) colonized simultaneously by C. gattii molecular type VGII and C. neoformans molecular type VNI, and a birdcage in a commercial aviary positive for C. neoformans, molecular type VNI. This is the first evidence of an environmental occurrence of molecular types VNI and VGII in PA.


Asunto(s)
Animales , Cryptococcus gattii/aislamiento & purificación , Cryptococcus neoformans/aislamiento & purificación , Vivienda para Animales , Árboles/microbiología , Aves , Brasil , Cryptococcus gattii/genética , Cryptococcus neoformans/genética , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA