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1.
J. venom. anim. toxins incl. trop. dis ; 15(2): 236-254, 2009. graf
Artículo en Inglés | LILACS | ID: lil-517293

RESUMEN

TsTX is an á-type sodium channel toxin that stimulates the discharge of neurotransmitters from neurons. In the present study we investigated which neurotransmitters are released in the hippocampus after TsTX injection and if they are responsible for electrographic or histopathological effects. Microdialysis revealed that the toxin increased glutamate extracellular levels in the hippocampus; however, levels of gamma-aminobutyric acid (GABA), glycine, 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were not significantly altered. Neurodegeneration in pyramidal cells of hippocampus and electroencephalographic alterations caused by the toxin were blocked by pretreatment with riluzole, a glutamate release inhibitor. The present results suggest a specific activity of TsTX in the hippocampus which affects only glutamate release.


Asunto(s)
Humanos , Animales , Ratas , Hipocampo , Neurotransmisores , Venenos de Escorpión/toxicidad
2.
J. venom. anim. toxins incl. trop. dis ; 13(2): 527-543, 2007. graf, tab
Artículo en Inglés | LILACS, SES-SP | ID: lil-452845

RESUMEN

Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32 percent glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29 percent valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.


Asunto(s)
Ratones , Animales , Antiinflamatorios , Aspergillus nidulans , Aspergilosis , Glicoproteínas
3.
J. venom. anim. toxins ; 8(1): 74-87, 2002. tab, graf
Artículo en Inglés | LILACS, SES-SP | ID: lil-303723

RESUMEN

Scorpion venom neurotoxins are responsible for toxicity and pharmacological effects. They are active in sodium and potassium channels leading to an increase in the release of neurotransmitters, such as glutamate. Glutamate is found in large quantities in the hippocampus (HPC) and is involved in the long-term potentiation (LTP) induction. The HPC is known to be related to certain kinds of memory. The aim of this study is to evaluate the effects of Tityus serrulatus TS-8F toxin on rat behavior with emphasis on learning and memory. We analyzed the effects of different doses of TS-8F on rat behavior in home cages, open-field (habituation), inhibitory avoidance, T-maze, and hippocampus morphology. In the first two experiments, 0.05µg/animal dose of TS-8F did not cause convulsion but led to a decrease in locomotion (LO) frequency in the open-field first session. During the second session, rats receiving 0.03µg/animal TS-8F showed a decrease in LO and rearing frequency (RE); controls only showed decreased LO; and those receiving 0.05µg/animal showed no significant changes. In inhibitory avoidance, T-maze, and HPC morphology experiments no significant differences were observed. It is concluded that TS-8F may exert some influence in rat learning and memory and seems to be useful as a pharmacological tool. Further research is required to elucidate all possible uses of this toxin.


Asunto(s)
Ratas , Animales , Masculino , Toxinas Biológicas , Aprendizaje , Memoria , Neurotoxinas , Venenos de Escorpión/efectos adversos , Venenos de Escorpión/toxicidad , Ratas Wistar , Convulsiones/inducido químicamente
4.
Braz. j. med. biol. res ; 34(11): 1397-1403, Nov. 2001. ilus, tab
Artículo en Inglés | LILACS, SES-SP | ID: lil-303314

RESUMEN

The opportunistic bacterium Proteus mirabilis secretes a metalloprotease, ZapA, considered to be one of its virulence factors due to its IgA-degrading activity. However, the substrate specificity of this enzyme has not yet been fully characterized. In the present study we used fluorescent peptides derived from bioactive peptides and the oxidized ß-chain of insulin to determine the enzyme specificity. The bradykinin- and dynorphin-derived peptides were cleaved at the single bonds Phe-Ser and Phe-Leu, with catalytic efficiencies of 291 and 13 mM/s, respectively. Besides confirming already published cleavage sites, a novel cleavage site was determined for the ß-chain of insulin (Val-Asn). Both the natural and the recombinant enzyme displayed the same broad specificity, demonstrated by the presence of hydrophobic, hydrophilic, charged and uncharged amino acid residues at the scissile bonds. Native IgA, however, was resistant to hydrolysis by ZapA


Asunto(s)
Proteus mirabilis , Metaloendopeptidasas , Proteínas Bacterianas , Proteus mirabilis , Espectrometría de Masas , Especificidad por Sustrato , Virulencia , Metaloendopeptidasas , Hidrólisis , Proteínas Bacterianas/análisis
5.
Braz. j. med. biol. res ; 33(7): 765-70, July 2000. tab, graf
Artículo en Inglés | LILACS, SES-SP | ID: lil-262675

RESUMEN

The protease ZapA, secreted by Proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. The control of its expression requires the use of an appropriate methodology, which until now has not been developed. The present study focused on the replacement of azocasein with fluorogenic substrates, and on the definition of enzyme specificity. Eight fluorogenic substrates were tested, and the peptide Abz-Ala-Phe-Arg-Ser-Ala-Ala-Gln-EDDnp was found to be the most convenient for use as an operational substrate for ZapA. A single peptide bond (Arg-Ser) was cleaved with a Km of 4.6 µM, a k cat of 1.73 s-1, and a catalytic efficiency of 376 (mM s)-1. Another good substrate for ZapA was peptide 6 (Abz-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-EDDnp) which was cleaved at a single bond (Phe-Ser) with a Km of 13.6 µM, a k cat of 3.96 s-1 and a catalytic efficiency of 291 (mM s)-1. The properties of the amino acids flanking the scissile bonds were also evaluated, and no clear requirement for the amino acid residue at P1 was found, although the enzyme seems to have a preference for a hydrophobic residue at P2.


Asunto(s)
Péptidos/análisis , Proteus mirabilis/enzimología , Proteínas Bacterianas , Metaloendopeptidasas , Endopeptidasas/aislamiento & purificación , Infecciones por Proteus/microbiología , Proteus mirabilis/genética , Proteus mirabilis/patogenicidad , Espectrometría de Fluorescencia , Espectrometría de Masas , Especificidad por Sustrato , Proteínas Bacterianas/análisis , Metaloendopeptidasas/análisis , Cinética , Caseínas/análisis , Hidrólisis
6.
J. venom. anim. toxins ; 6(2): 238-60, 2000. tab, graf
Artículo en Inglés | LILACS, SES-SP | ID: lil-276611

RESUMEN

It has been previously shown that the crude venom of Tityus serrulatus can cause convulsions. This study was designed to investigate the neurotoxic effects of B, C, G, and K fractions isolated from this venom. Intravenous injection of these fractions in mice (0.6 - 6.0 mg/kg body weight) showed that the C fraction is a potent convulsant and G fraction decreased the threshold for tonic hand limb extension elicited by transauricular electroshock. Unilateral injection of B, C, and K fractions, but not G fraction, into the spikes and epileptic discharges that began in the hippocampus and evolved to the cortex. The following motor signs were observed: movements of facial muscles, wet dog shake, immobility, myoclonus, wild-running with clonus, and in some cases, loss of postural control. Intrahippocampal injection of B, C, and K fraction, but not G fraction, caused neuronal loss at the injection site as well as in other hippocampal areas. The effect of these fractions on epileptiform activity and on neuronal loss was dose-dependent. The severity of the epileptiform activity in the ipsilateral hippocampus correlated with the severity of the neuronal loss. The electrographic, behavioral, and histological changes induced by b, C, and K fractions were similar to those obtained with other drugs that are commonly used to induce convulsion. The convulsant effects of the crude venom may be caused by the fractions studied in this work.


Asunto(s)
Masculino , Ratas , Ratones , Conducta Animal/efectos de los fármacos , Electroencefalografía , Hipocampo/efectos de los fármacos , Convulsiones/inducido químicamente , Venenos de Escorpión/toxicidad , Convulsiones/inducido químicamente , Estudios de Casos y Controles , Ratas Wistar , Análisis de Varianza , Relación Dosis-Respuesta a Droga , Venenos de Escorpión/aislamiento & purificación
7.
Braz. j. med. biol. res ; 21(3): 645-7, Mar. 1988. tab
Artículo en Inglés | LILACS | ID: lil-60271

RESUMEN

The effects of icv administration of ß-endorphin on secretory activity of dopaminergic neurons is described. Homovanillic and dihydroxyphenyl acetic acid levels in cerebrospinal fluid and extracts of brain tissue were determined after administration of ß-endorphin to animals pretreated or not with saloxone. The results suggest that ß-endorphin interferes with formation of dopaminergic metabolites by acting on opioid receptors


Asunto(s)
Ratas , Animales , Ácido 3,4-Dihidroxifenilacético/líquido cefalorraquídeo , betaendorfina/fisiología , Cuerpo Estriado/metabolismo , Ácido Homovanílico/líquido cefalorraquídeo , Sustancia Negra/metabolismo , Dopamina/metabolismo , Naloxona/uso terapéutico , Neuronas/fisiología
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