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Braz. j. infect. dis ; Braz. j. infect. dis;18(5): 501-506, Sep-Oct/2014. tab, graf
Artículo en Inglés | LILACS | ID: lil-723085

RESUMEN

Background: Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection. Methods: The murine intracranial abscesses model induced by S. aureus was constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay. Results: In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model. Conclusion: Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice. .


Asunto(s)
Animales , Femenino , Absceso Encefálico/microbiología , Genes Bacterianos , Genes Reguladores , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/patogenicidad , Absceso Encefálico/mortalidad , Absceso Encefálico/patología , Modelos Animales de Enfermedad , Infecciones Estafilocócicas/mortalidad , Infecciones Estafilocócicas/patología , Staphylococcus aureus/genética , Virulencia
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