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1.
National Journal of Andrology ; (12): 613-617, 2018.
Artículo en Chino | WPRIM | ID: wpr-689711

RESUMEN

<p><b>Objective</b>To study the influence of povidone-iodine (PI) versus that of the benzethonium chloride wipe (BCW) on semen collection and semen quality of sperm donors undergoing penile skin disinfection and provide some evidence for the selection of disinfection methods for semen collection.</p><p><b>METHODS</b>We used PI from August to December 2015 and BCWs from January to July 2016 for penile skin disinfection before semen collection, with two samples from each donor, one collected with and the other without penis skin disinfection (the blank control group). After semen collection, we conducted a questionnaire investigation on the influence of the two disinfection methods on semen collection and compared the semen parameters between the two groups of sperm donors.</p><p><b>RESULTS</b>Totally, 185 sperm donors were included in this study, of whom 63 underwent penile skin disinfection with PI and the other 122 with BCWs before semen collection. Statistically significant differences were found between the PI and BCW groups in the adaptability to the disinfectant and rigid disinfection procedures (P <0.05), but not in the other items of the questionnaire (P >0.05). Compared with the sperm donors of the blank control group, those of the PI group showed statistically significant difference in the percentage of progressively motile sperm (PMS) ([63.02 ± 3.18]% vs [61.45 ± 4.78]%, P<0.05), but not in the abstinence time ([4.97 ± 1.79] vs [4.7 ± 0.94] d, P >0.05), semen volume ([4.11 ± 1.54] vs [4.15 ± 1.61] ml, P >0.05), sperm concentration ([110 ± 29.6] vs [107.5 ± 31.79] ×10⁶/ml, P >0.05), or total sperm count ([439.10 ± 170.13] vs [434.02 ± 186.91] ×106/ejaculate, P >0.05), while those of the BCW group exhibited no remarkable difference in any of the above parameters (P >0.05). Among the samples with abnormal semen quality, significantly fewer were found with abnormal PMS in the BCW than in the PI group (1.64% [2/122] vs 9.68% [6/62], P <0.05). However, there were no significant differences between the PI and BCW groups in the abnormal semen volume, abnormal sperm concentration, or the rate of semen bacterial contamination (P >0.05).</p><p><b>CONCLUSIONS</b>Before semen collection from donors, penile skin disinfection with povidone-iodine may affect both the semen collection process and the quality of donor sperm, while the benzethonium chloride wipe can reduce the influence on the semen collection process and does not affect the semen parameters.</p>


Asunto(s)
Humanos , Masculino , Antiinfecciosos Locales , Bencetonio , Desinfección , Métodos , Pene , Povidona Yodada , Semen , Análisis de Semen , Piel , Recuento de Espermatozoides , Recuperación de la Esperma , Espermatozoides , Donantes de Tejidos
2.
Chinese Journal of Preventive Medicine ; (12): 352-357, 2013.
Artículo en Chino | WPRIM | ID: wpr-274714

RESUMEN

<p><b>OBJECTIVE</b>To study the gene expression patterns in livers of infant rats after Benzo[a]pyrene (BaP) exposure during pregnancy and explore the important gene and signaling pathways in the toxic mechanism of BaP.</p><p><b>METHODS</b>Thirty-two pregnant SD rats were randomly divided into four groups: vehicle control (corn oil) and treatment groups (0.75, 1.50 and 3.00 mg/kg BaP in corn oil). BaP solutions were given by gastric infusion from the 3rd to the 17th day of pregnancy. After delivery the offspring's liver were taken to detect the gene expression by RatRef-12 gene chip. The stability of gene chip was tested by repeated experiments.</p><p><b>RESULTS</b>After prenatal BaP exposure 1232 genes with different expression variations in hepatocytes of offsprings were identified. Three expression patterns of genes related to the dose of prenatal BaP exposure were identified with significant difference (P < 0.05). As the dose of prenatal BaP exposure increased, the gene expression patterns were downregulated, upregulated, and fluctuated. Twenty-six signaling pathways with differently expressed genes mainly focused on: growth and development, toxicant metabolism and inflammation (P < 0.05). The data from gene network analysis demonstrated that CYP2C13, GSTO1, Rela, MAPK8 and Plcg1 were the key genes in the gene network.</p><p><b>CONCLUSION</b>Gene expression patterns of offsprings' hepatocytes were influenced by prenatal BaP exposure. Some key genes and signal pathways were also found. The study provides an important clue for the toxicity and mechanisms of the prenatal BaP exposure on the growth and development of offspring.</p>


Asunto(s)
Animales , Femenino , Masculino , Embarazo , Ratas , Benzo(a)pireno , Toxicidad , Expresión Génica , Hepatocitos , Análisis de Secuencia por Matrices de Oligonucleótidos , Efectos Tardíos de la Exposición Prenatal , Genética , Ratas Sprague-Dawley , Transducción de Señal
3.
Acta Pharmaceutica Sinica ; (12): 452-458, 2012.
Artículo en Chino | WPRIM | ID: wpr-323020

RESUMEN

This study is to investigate the anti-tumor effect in vitro of methotrexate modified by LH-RH peptide (LH-RH-MTX). LH-RH receptors highly expressing MCF-7 human breast carcinoma cell line and lowly expressing K562 human erythroleukemia cell line were served as the tested cells. The cell proliferation inhibition rates of LH-RH-MTX were detected by MTT colorimetric assay. The effects of LH-RH-MTX on the cell cycle and apoptosis rates were detected by flow cytometry. The inhibition rate of LH-RH-MTX on MCF-7 cells was much higher than that on K562 cells, and the inhibition rate of LH-RH-MTX on MCF-7 cells was much higher than that of free MTX at the same concentration. The inhibition rate of LH-RH-MTX on rat bone marrow mononuclear cells was less than that of free MTX. The number of MCF-7 cells in S phase increased after administration of LH-RH-MTX. The apoptosis rate of LH-RH-MTX group significantly increased compared with that of the control group and MTX group. The relative expression of LHRHR mRNA of LH-RH-MTX group markedly decreased compared with that of the control group and MTX group. LH-RH-MTX is realizable to reduce drug side effects, increase the therapeutic index and achieve tumor-targeted therapy.


Asunto(s)
Animales , Humanos , Ratas , Antimetabolitos Antineoplásicos , Farmacología , Apoptosis , Células de la Médula Ósea , Biología Celular , Ciclo Celular , Proliferación Celular , Células Cultivadas , Sistemas de Liberación de Medicamentos , Hormona Liberadora de Gonadotropina , Química , Farmacología , Células K562 , Leucocitos Mononucleares , Células MCF-7 , Metotrexato , Farmacología , ARN Mensajero , Metabolismo , Receptores LHRH , Genética
4.
Chinese Journal of Preventive Medicine ; (12): 371-375, 2009.
Artículo en Chino | WPRIM | ID: wpr-242648

RESUMEN

<p><b>OBJECTIVE</b>To explore the public health situation and needs in Anxian after Wenchuan earthquake so as to make an effective strategy for disease control and prevention.</p><p><b>METHODS</b>69 concentrated settlements with 100 residents were investigated. Probability proportion to size was adopted for sampling of 2200 residents from 687 scattered households (about 440 000 scattered residents). The content of this survey included drinking water, food hygiene, environment sanitation, planning immunity and medical health service, disease surveillance and so on. SPSS 16.0 was used for data analysis, and statistical interpretation was used to describe the results.</p><p><b>RESULTS</b>90.9% (31/66) resettled residents in Anxian lived in tents, 7.6% (5/66) lived in the movable-plate house, 93.3% (621/666) scattered households lived in tents and 71.9% (446/621) of them lived in tents which were built by residents themselves; the rate of drinking water disinfection in resettlement sites and scattered households were 97.1% (66/68) and 94.6% (650/687); 12.8% scattered residents had mouldy or food; 50% of resettlement sites raised animals; 43.6% (17/39) medical station didn't have bacterin inoculation service; 66.7% (10/15) lacked sufficient disinfection equipment; register rate was 50.0% (33/66) and report rate of symptoms and infectious diseases was 56.1% (37/66).</p><p><b>CONCLUSION</b>There was still some risk of enteric and vector-borne diseases in Anxian, therefore, some tailored measures should be very important.</p>


Asunto(s)
Humanos , Desastres , Terremotos , Monitoreo del Ambiente , Encuestas de Atención de la Salud , Servicios de Salud , Necesidades y Demandas de Servicios de Salud , Abastecimiento de Agua
5.
Chinese Journal of Preventive Medicine ; (12): 380-384, 2009.
Artículo en Chino | WPRIM | ID: wpr-242646

RESUMEN

<p><b>OBJECTIVE</b>To explore the mental health status of residents scattered living in Anxian after Wenchuan earthquake so as to provide scientific basis for further mental health intervention.</p><p><b>METHODS</b>A face to face interview was conducted among the scattered residents with designed questionnaire, which had three parts of the physical and emotional reaction, the relax methods and the social care and supports expected. Two-stage probability proportional to size (PPS) sample method was performed to sample 2184 from 0.44 million scattered residents in Anxian. On the basis of statistical description, mental health of different characteristics groups was compared.</p><p><b>RESULTS</b>Three main symptoms of posttraumatic stress disorders in 2184 residents (11.23+/-3.44) were higher than the 103 fire victims in Hunan in 2003 (10.06+/-3.26), three factor scores of SCL-90 (5.76+/-1.74) were higher than normal in 1998 repair mode (n=23 891) (4.72+/-1.44), and the statistical difference was observed (t=10.77, P<0.05; t=706.04, P<0.05). Comparing the mental health of different groups, some significant differences were found by age, gender and education background.</p><p><b>CONCLUSION</b>The earthquake disaster brought prevalent physical and emotional reaction. Elderly people, female, junior students need mental intervention immediately. Therefore, strengthen the mental education and assistance (especially in high risk groups) would be of more significance.</p>


Asunto(s)
Adolescente , Adulto , Niño , Preescolar , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Persona de Mediana Edad , Adulto Joven , Desastres , Terremotos , Salud Mental , Muestreo , Trastornos por Estrés Postraumático , Epidemiología , Psicología , Encuestas y Cuestionarios
6.
Journal of Zhejiang University. Science. B ; (12): 20-27, 2006.
Artículo en Inglés | WPRIM | ID: wpr-263229

RESUMEN

Twenty-three temperate China species of Lachnum, Lachnum abnorme, L. angustum, L. brevipilosum, L. calosporum, L. calyculiforme, L. carneolum, L. ciliare, L. controversum, L. flavidulum, L. cf. fushanese, L. indicum, L. kumaonicum, L. lushanese, L. minutum, L. montanum, L. cf. pteridophyllum, L. pygmaeum, L. sclerotii var. sclerotii, L. sclerotii var. sichuanense, L. subpygmeaum, L. tenuissimum, L. virgineum and L. willisii are reported, whose main characteristics are given in a formula of the described species, some of which are discussed below.


Asunto(s)
Ascomicetos , Clasificación , Biología Celular , Biodiversidad , China , Clima , Especificidad de la Especie
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