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1.
Chinese Journal of Experimental and Clinical Virology ; (6): 112-113, 2011.
Artículo en Chino | WPRIM | ID: wpr-231178

RESUMEN

<p><b>OBJECTIVE</b>To investigate the correlation of sera HBV DNA and serological makers with hepatic tissue HBVcccDNA in chronic HBV carriers.</p><p><b>METHODS</b>Real time fluorescence quantitative polymerase chain reaction (RT-PCR) were used to detect HBV covalently closed circular DNA (cccDNA) and total intrahepatic HBV DNA from 30 needle-biopsy specimens as well as HBV DNA in sera in chronic HBV carriers. Quantification of the HBsAg, HBeAg in sera were quantified using Chemiluminescence immunoassay.</p><p><b>RESULTS</b>HBVcccDNA can be detected in chronic HBV carriers, which rang from 3.15 x 10(3) copies/mg to 1.06 x 10(7) copies/mg. There was a positive correlation between the cccDNA and HBVtDNA (r = 0.375, P < 0.05), but there was no correlation between the cccDNA and sera HBV DNA (P = 0.174). There was a positive correlation between cccDNA and sera HBsAg quantification (r = 0.562, P < 0.001) but no correlation with sera HBeAg qantification (r = 0.152, P > 0.05).</p><p><b>CONCLUSION</b>HBV cccDNA can be replicated stably in hepatic tissue in all chronic HBV carriers. HBV DNA in sera can not be indicated hepatic tissue cccDNA level. While HBsAg quantification in sera can be used as a marker of cccDNA quantification in hepatic tissue to some extent.</p>


Asunto(s)
Adolescente , Adulto , Femenino , Humanos , Masculino , Adulto Joven , Biopsia , Portador Sano , Sangre , Patología , Virología , ADN Circular , Genética , ADN Viral , Sangre , Genética , Virus de la Hepatitis B , Genética , Hepatitis B Crónica , Sangre , Patología , Virología , Hígado , Patología , Virología
2.
Chinese Journal of Experimental and Clinical Virology ; (6): 344-347, 2004.
Artículo en Chino | WPRIM | ID: wpr-279539

RESUMEN

<p><b>OBJECTIVE</b>To identify the location of major immunodominant antigenic region and study the relationship between the gene heterogeneity and immunoreactivity via detecting antigenic reactivity of synthetic peptides deriving from immunodominant region in different genotypes of hepatitis C virus (HCV) NS5a gene.</p><p><b>METHODS</b>In total, 305 non-identical 30-mer long and overlapping by 15 aa peptides derived from HCV NS5a region from codon 2,182 to 2,343 among 45 unique published HCV sequences in GenBank corresponding to different genotype were designed and synthesized. The amino acid sequences of all peptides were compared with DNA Star software. The antigenic reactivity of those peptides was detected with indirect ELISA with both anti-HCV and anti-NS5 positive serum.</p><p><b>RESULTS</b>The sequences showed highly conserved among HCV genotype in regions 2,272-2,301 and 2,302-2,331 as compared to regions 2,212-2,241 and 2,257-2,286. The peptides basing on amino acid residues among 2,212-2,241, 2,272-2,301 and 2,302-2,331 showed stronger immunoreactivity than any other peptides. Eighteen peptides derived from this region showed a broad immunoreactivity, 3 of them could react with 96% of anti-HCV positive sera. Whereas the immunoreactivity of the peptides derived from the region showing highly variable among HCV genotype was found to react more strongly with homologous-genotype sera.</p><p><b>CONCLUSION</b>The major linear antigenic region was located at amino acid residues among 2,212-2,241, 2,272-2,301 and 2,302-2,331; the short synthetic peptide derived from NS5a region at position 2,212-2,241, 2,272-2,301 and 2,302-2,331 can be used for efficient detection of HCV antibody; some peptides showed genotype specific immuunoreactivity.</p>


Asunto(s)
Humanos , Secuencia de Aminoácidos , Variación Antigénica , Antígenos Virales , Sangre , Alergia e Inmunología , Mapeo Epitopo , Genotipo , Hepacivirus , Clasificación , Genética , Alergia e Inmunología , Hepatitis C , Alergia e Inmunología , Virología , Anticuerpos contra la Hepatitis C , Sangre , Alergia e Inmunología , Epítopos Inmunodominantes , Péptidos , Alergia e Inmunología , Proteínas Recombinantes , Genética , Alergia e Inmunología , Homología de Secuencia de Aminoácido , Proteínas no Estructurales Virales , Genética , Alergia e Inmunología
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