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1.
Chinese Journal of Pathology ; (12): 20-22, 2012.
Artículo en Chino | WPRIM | ID: wpr-242005

RESUMEN

<p><b>OBJECTIVE</b>To compare the detection sensitivity of epidermal growth factor receptor (EGFR) mutations between allele specific oligonucleotide PCR (ASO-PCR) and bi-loop probe and specific primer quantitative PCR (BPSP-qPCR).</p><p><b>METHODS</b>A total of 96 non-small cell lung cancer specimens were selected from West China Hospital from September 2009 to December 2010. ASO-PCR was developed to detect the presence of classical EGFR mutations. A total 39 available specimens were also tested by BPSP-qPCR.</p><p><b>RESULTS</b>EGFR mutation detection rate was 30.2% (26/96) by ASO-PCR. The mutation rate was higher in female than in male patients [45.5% (20/44) vs. 17.3% (9/52), P = 0.003], non-smokers than smokers [44.1% (26/59) vs. 8.1% (3/37), P < 0.001] and adenocarcinomas than other subtypes of lung cancer [37.0% (27/73) vs. 8.7% (2/23), P = 0.01]. Among mutation negative cases by ASO-PCR, BPSP-qPCR increased the rate of detection of 19-del and L858R mutation by 10.3% (4/39) in adenocarcinomas and non-smoking subset. Overall, the mutation detection rate of BPSP-qPCR was higher than that of ASO-PCR [66.7% (26/39) vs. 41.0% (16/39), P = 0.02].</p><p><b>CONCLUSION</b>BPSP-qPCR has a better detection sensitivity than that of ASO-PCR.</p>


Asunto(s)
Femenino , Humanos , Masculino , Persona de Mediana Edad , Adenocarcinoma , Genética , Carcinoma de Pulmón de Células no Pequeñas , Genética , Análisis Mutacional de ADN , Genes erbB-1 , Neoplasias Pulmonares , Genética , Mutación , Reacción en Cadena de la Polimerasa , Métodos , Receptores ErbB , Genética , Sensibilidad y Especificidad , Factores Sexuales , Fumar
2.
Chinese Journal of Pathology ; (12): 667-670, 2011.
Artículo en Chino | WPRIM | ID: wpr-358268

RESUMEN

<p><b>OBJECTIVE</b>To investigate the sensitivity of bi-loop probe and specific primer quantitative PCR (BPSP-qPCR) in the detection of epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>BPSP-qPCR was employed to examine the presence of mutations of EFGR exon 19 through 21. Correlation of the mutations with clinicopathological characteristics and types of tumor samples were performed.</p><p><b>RESULTS</b>In the cohort of 265 specimens, 30.2% (80/265) mutations were found to be 19-del and/or L858R. Females (39.7%, 31/78), non-smokers (41.0%, 43/105) and adenocarcinoma patients (37.8%, 51/135) had a higher mutation rate (P<0.05) among 184 patients whose profiles were available. T790M combined with 19-del and/or L858R accounted for 3.3% (6/184) of the mutations. Male metastatic tumors (29.6%, 8/27), pleural fluids of females (42.9%, 9/21) and non-smokers (40.7%, 11/27) were found to have higher percentage of 19-del and/or L858R mutations, in contrast, no mutations were found in the metastatic lesions of non-adenocarcinoma patients (P>0.05).</p><p><b>CONCLUSIONS</b>BPSP-qPCR is a robust method in detection of EGFR mutations with high consistency and sensitivity. The difference of EGFR mutations in primary tumors, metastatic lesions and pleural fluids suggests that EGFR tyrosine kinase inhibitors (EGFR-TKI) treatment may have variable treatment effects depending on the tumor sites.</p>


Asunto(s)
Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adenocarcinoma , Genética , Carcinoma de Pulmón de Células no Pequeñas , Genética , Patología , Exones , Eliminación de Gen , Genes erbB-1 , Neoplasias Pulmonares , Genética , Patología , Mutación , Tasa de Mutación , Derrame Pleural Maligno , Genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Métodos , Receptores ErbB , Genética , Sensibilidad y Especificidad , Factores Sexuales , Fumar
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