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Indian J Biochem Biophys ; 2008 Aug; 45(4): 275-7
Artículo en Inglés | IMSEAR | ID: sea-28081

RESUMEN

Synechocystis sp. PCC 6803 lacks a gene for the any known types of lycopene cyclase. Recently, we reported that Sll0659 (unknown for its function) from Synechocystis sp. PCC6803 shows similarity in sequence to a lycopene cyclase gene-CruA from Chlorobium tepidum. To test, whether sll0659 encoded protein serves as lycopene cyclase, in this study, we investigated the carotenoids of the wild types and mutants. In the sll0659 deleted mutant, there is no blockage at the lycopene cyclization step. Our results demonstrate that sll0659 does not affect lycopene cycilzation. However, the ultrastructure of mutants suggests the involvement or necessity of sll0659 in the cell division.


Asunto(s)
Proteínas Bacterianas/genética , Secuencia de Bases , División Celular/genética , Chlorobium/enzimología , Cromatografía Líquida de Alta Presión , Clonación Molecular , Ciclización , Electroforesis en Gel de Poliacrilamida , Genes Bacterianos , Liasas Intramoleculares/genética , Microscopía Electrónica de Transmisión , Mutación , Reacción en Cadena de la Polimerasa , Synechocystis/citología , beta Caroteno/metabolismo
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