Establishment of a human large cell lung cancer cell line L9981-nm23-H1 / 中国肺癌杂志

<p><b>BACKGROUND</b>To establish a human large cell lung cancer cell line L9981-nm23-H1 transfected with wild type nm23-H1 gene.</p><p><b>METHODS</b>pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and L9981-nm23-H1 was established by infected virus of nm23-H1 gene. The DNA and protein expression of nm23-H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot.</p><p><b>RESULTS</b>pLXSN-nm23-H1-EGFP was constructed successfully, and the nm23-H1 cDNA was inducted to L9981 cell. The protein of nm23-H1 could be detected in L9981-nm23-H1 cell.</p><p><b>CONCLUSIONS</b>Protein of nm23-H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.</p>