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Journal of Central South University(Medical Sciences) ; (12): 854-860, 2010.
Artículo en Chino | WPRIM | ID: wpr-814384

RESUMEN

OBJECTIVE@#To establish 2-dimensional electrophoresis (2-DE) graph of A549 and A549/DDP cell lines, to identify the differentially expressed proteins, and to screen multidrug resistance (MDR) related proteins in human lung adenocarcinoma.@*METHODS@#The total proteins of A549 and A549/DDP cells were obtained, and were extracted and separated by 2-DE. PDQuest software was applied to analyze the 2-DE images, and the differential proteins of the 2 types of cells were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Western blot was used to determine the expression levels of the 4 proteins.@*RESULTS@#We established 2-DE maps of total proteins from A549 and A549/DDP. A total of 40 differential protein spots in the 2 cell lines were found, and 23 differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that heat shock protein beta-1, annexin A4, cofilin l, vimentin were differential expression proteins in A549 and A549/DDP, which was consistent with the results of the comparative proteomic analysis.@*CONCLUSION@#The 23 differential expression proteins in human lung adenocarcinoma are useful for studying the MDR mechanism of lung adenocarcinoma.


Asunto(s)
Humanos , Adenocarcinoma , Genética , Metabolismo , Patología , Anexina A4 , Antineoplásicos , Farmacología , Línea Celular Tumoral , Resistencia a Múltiples Medicamentos , Genética , Resistencia a Antineoplásicos , Genética , Electroforesis en Gel Bidimensional , Proteínas de Choque Térmico HSP27 , Neoplasias Pulmonares , Genética , Metabolismo , Patología , Proteínas de Neoplasias , Clasificación , Proteoma , Proteómica , Métodos
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