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Objective To study the relations between CD23 expression on peripheral blood B lymphocytes and IgE in asthmatic children and their pathogenesis. Methods CD19 +, CD23 +, CD19 +/CD23 + expressions of peripheral blood lymphocytes were measured by flow cytometry and serum total IgE level by chemiluminescence immunoassay in 22 children with classical asthma, 18 cough variant asthma (CVA), 20 with asthmatiod bronchitis ,20 with pneumonia and 25 healthy controls. The correlations between CD23 expression on peripheral blood B lymphocytes and IgE in serum were analysed. The 20 children with asthmatiod bronchitis were divided into two groups by the scores whether it reached that for diagnosing asthma after ten months: Group Ⅰ (10 cases) and Group Ⅱ (10 cases). Results CD19 +, CD23 +, CD19 +/CD23 + expressions on lymphcytes were significantly elevated in peripherial blood and serum IgE from children with classical asthma [(13.56?5.87)%,(26.56?7.61)%,(12 86?5.01)%, (12.67?7.56) ?mol/L respectively] and CVA [(13.10?5.15)% ,(24.66?8.15)% , (12 10?4.97)%, (9.45?4.16) ?mol/L respectively] as compared with those in the children with pneumonia and healthy controls [(7.95?3.65)%, (14.92?3.02)% ,(6.82?2.95)% , (3.10?1.68) ?mol/L respectively] ( P
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Objective:To supply a reference measure,CD8+CD38+ expressin,AST,TBIL,PT were examined in patients with chronic hepatitis B.Methods:CD8+CD38+ expression of peripheral blood lymphocyte in 38 patients with chromic hepatitis B and 14 patients with liver cirrhosis were detected with flow cytometry. AST,TBIL,PT were also measured at the same time with instruments.Results:(1)CD8+CD38+ expression in patients with choronic hepatitis B and liver cirrcosis elevated significantly compared with those in normal controls. But CD8+CD38+ expression in patients with liver cirrhosis was lower significantly than those with chronic hepatitis B.(2)CD8+CD38+ expression in moderate chronic hepatitis B were higher than those in mild chronic hepatitis B. CD8+CD38+,CD8+,CD38+ expression in severe chronic hepatitis B were higher than those in mild chronic hepatitis B. CD38+ expression in severe chronic hepatitis B was higher than those in moderate chronic hepatitis B. CD8+CD38+,CD8+,CD38+ expression in liver cirrhosis was lower than those in mild,moderate,severe chronic hepatitis B. CD8+ expression in liver cirrhosis was lower than those in severe chronic hepatitis B.(3)CD8+CD38+ expression in patients under AST,TBIL,PT abnormal condition were higher than those in patients under AST,TBIL PT,normal condition.Conclusion:The increasing of CD8+CD38+ expression of peripheral blood lymphocyte in patients with chronic hepstitis B,reflect the fact that cell immunolgy was abnormally activated.There was a remarkable correlation on CD8+CD38+ and AST,TBIL,PT. To test expression of CD8+CD38+ of peripheral blood lymphocyte in chronic hepatitis B has very important clinical reference value on patient′s condition development and diagnosis.
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0.05).There was a positive corrlation on CD40~+ and CD40L~+ in chronic B hepatitis patients.There was a positive corrlation on CD8~+/CD28~+ and CD40~+、CD40L~+ in chronic B hepatitis patients.There were no remarkable corrlation on CD8~+/CD28~- and CD40~+、CD40L~+ in chronic B hepatitis patients.Conclusion:The costimulation molecules CD40~+、CD40L~+ and CD8~+/CD28~+ are lower,while CD8~+/CD28~- are higher in chronic B hepatitis patients than in the health.To test the expression of CD40~+、CD40L~+ and CD8~+/CD28~+ on peripheral blood of the chronic B hepatitis could help to evaluate patients's celluar immunity and guide clinical treatment.