Determination of bioactive components in the fruits of Cercis chinensis Bunge by HPLC-MS/MS and quality evaluation by principal components and hierarchical cluster analyses / 药物分析学报

The fruits of leguminous plants Cercis Chinensis Bunge are still overlooked although they have been reported to be antioxidative because of the limited information on the phytochemicals of C.chinensis fruits.A simple,rapid and sensitive HPLC-MS/MS method was developed for the identification and quantitation of the major bioactive components in C.chinensis fruits.Eighteen polyphenols were iden-tified,which are first reported in C.chinensis fruits.Moreover,ten components were simultaneously quantified.The validated quantitative method was proved to be sensitive,reproducible and accurate.Then,it was applied to analyze batches of C.chinensis fruits from different phytomorph and areas.The principal components analysis (PCA) realized visualization and reduction of data set dimension while the hierarchical cluster analysis (HCA) indicated that the content of phenolic acids or all ten components might be used to differentiate C.chinensis fruits of different phytomorph.

Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS/MS / 药物分析学报

The roots of O.fragrans are also a valuable resource in addition to its flowers and fruits.In this study,the HPLC-MS/MS method used for analyzing the chemical constituents in O.fragrans roots extract was developed,which showed high sensitivity for both qualitative and quantitative analyses.Thirty-two compounds were first discovered in O.fragrans roots,one compound of which was reported for the first time.The simultaneous determination method for acteoside,isoacteoside,oleuropein and phillyrin was validated to be sensitive and accurate.Then it was applied to determine the content of bioactive components in O.fragrans roots from different cultivars.The content of oleuropein and phillyrin in the twelve batches was relatively stable,while the content of acteoside and isoacteoside varied greatly.Moreover,the therapeutic material basis and mechanism of O.fragrans roots exerting its traditional pharmacodynamics were analyzed by network pharmacology.The results showed that O.fragrans roots might be effective for the treatment of inflammation,cardiovascular diseases,cancer,and rheumatoid arthritis,which is consistent with the traditional pharmacodynamics of O.fragrans roots.This work can provide an analytical method for the comprehensive development of O.fragrans roots.