Construction and structural analysis of integrated cellular network of Corynebacterium glutamicum / 生物工程学报

Corynebacterium glutamicum is one of the most important traditional industrial microorganisms and receiving more and more attention towards a novel cellular factory due to the recently rapid development in genomics and genetic operation toolboxes for Corynebacterium. However, compared to other model organisms such as Escherichia coli, there were few studies on its metabolic regulation, especially a genome-scale integrated cellular network model currently missing for Corynebacterium, which hindered the systematic study of Corynebacterium glutamicum and large-scale rational design and optimization for strains. Here, by gathering relevant information from a number of public databases, we successfully constructed an integrated cellular network, which was composed of 1384 reactions, 1276 metabolites, 88 transcriptional factors and 999 pairs of transcriptional regulatory relationships. The transcriptional regulatory sub-network could be arranged into five layers and the metabolic sub-network presented a clear bow-tie structure. We proposed a new method to extract complex metabolic and regulatory sub-network for product-orientated study taking lysine biosynthesis as an example. The metabolic and regulatory sub-network extracted by our method was more close to the real functional network than the simplex biochemical pathways. The results would be greatly helpful for understanding the high-yielding biomechanism for amino acids and the re-design of the industrial strains.

Latent fire theory / 中华中医药杂志

In Key to Therapeutics of Children,s Diseases, compiled by QIAN Yi in Song Dynasty, Xiebaisan and Xiehuangsan were used to treat heat syndrome of zang-fu. According to that academic thinking, the later generations proposed the theory of latent fire, yin f ire, stagnant fire, and the latent fire theory was the most prevalent. In clinics, the author treated chronic infectious diseases with latent fire theory, it achieved obvious curative effect. This article discussed his clinical experience from the aspects of literature analysis, identification of concept, development of pathogenesis, symptoms and signs, medication feature.