Clinical observation of warm acupuncture combined with external application of Tibetan medicine Baimai Ointment in the treatment of low-back pain with cold-dampness type / 国际中医中药杂志

Objective:To evaluate the clinical efficacy of warm acupuncture combined with external application of Tibetan medicine Baimai Ointment in the treatment of low-back pain with cold-dampness type.Methods:Randomized controlled trial. Totally 60 outpatients in Tibetan Medicine Hospital of Cuona County from May to July of 2021 were selected as the observation objects, and they were divided into two groups by random number table method, with 30 cases in each group. The control group was treated with Baimai Ointment, and the treatment group was treated with warm acupuncture and Baimai Ointment. Both groups were treated for 2 weeks and followed up for 3 months. VAS scale and Oswestry disability index (ODI) were used to evaluate the low-back pain and dysfunction, and the clinical efficacy was evaluated.Results:The VAS scores of the treatment group were lower than those in the control group immediately after treatment and at the last follow-up ( t=-18.17, -6.05, P<0.01). The ODI score of the treatment group was lower than that of the control group at the last follow-up ( t=-15.86, P<0.01). The total effective rate was 96.7% (29/30) in the treatment group and 93.3% (28/30) in the control group, without statistical significance ( χ2=0.001, P=1.000). Conclusion:Warm acupuncture combined with Tibetan medicine Baimai Ointment can effectively improve the clinical symptoms of low-back pain with cold-dampness type, improve the quality of life of patients, and the clinical effect is satisfactory.

The short-term and medium-term outcomes of children with type-Ⅲ congenital esophageal atresia after surgery / 中华胸心血管外科杂志

Objective:To analyze the short-term and medium-term survival status of children with congenital esophageal atresia, and to provide reference for clinical multidisciplinary management of children with congenital esophageal atresia.Methods:The clinical data of neonates with type Ⅲ congenital esophageal atresia who were operated in our hospital between November 2007 to November 2018 and followed up in this hospital were analyzed retrospectively.Results:Among the 62 cases, 16 cases were discharged automatically, 1 case died, and 45 cases were included in the short-term follow-up. 35 cases were classified as gross Ⅲa, 10 as Ⅲb, 5 as long segment type, 44 patients accepted one-stage surgery, 1 infant accepted delayed operation, 9 infants received second operations. Anastomotic leakage occurred in 8 cases (17.8%), anastomotic stenosis in 11 cases (24.4%), recurrence of tracheoesophageal fistula in 2 cases (4.4%), blood flow infection in 14 cases(31.1%), incision infection in 4 cases (8.9%). The medium-term survival status of 38 cases: 2 cases died of aspiration, 29 cases (76.3%) of anastomotic stenosis underwent esophageal dilatation, 5 cases (13.2%) of dysphagia when 1.5 years old, 6 cases (15.8%) of malnutrition. After multidisciplinary collaboration, the survival rate increased (57.1% vs. 85.3%, P=0.013), and the incidence of anastomotic leakage decreased (46.4% vs. 20.6%, P=0.03). Conclusion:The quality of life of children with congenital esophageal atresia can be improved by multidisciplinary cooperation and standardized postoperative follow-up.

miR-1301/TRIAP1 Axis Participates in Epirubicin-Mediated Anti-Proliferation and Pro-Apoptosis in Osteosarcoma

PURPOSE: Epirubicin is one of the most effective drugs against osteosarcoma. miR-1301 is involved in the occurrence and development of osteosarcoma. Whether miR-1301 is responsible for the chemosensitivity of osteosarcoma cells to epirubicin remains largely unknown. MATERIALS AND METHODS: U2OS and SAOS-2 cells were treated with various concentrations of epirubicin. Flow cytometry was employed to evaluate cell apoptotic rate. Cell proliferation was measured by Cell Counting Kit-8 assay. Western blot and quantitative real-time polymerase chain reaction were utilized to detect the expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 assaciated X protein (Bax), cleaved-caspase-3, cleaved-poly (ADP-ribose) polymerases (PARP1), TP53-regulated inhibitor of apoptosis 1 (TRIAP1), and microRNA-1301 (miR-1301). The relationship between miR-1301 and TRIAP1 was determined by luciferase reporter assay. RESULTS: Epirubicin inhibited proliferation in a dose-dependent manner, induced apoptosis, decreased the expression of Bcl-2, and increased the expressions of Bax, cleaved-caspase-3, and cleaved-PARP1 in osteosarcoma cells. miR-1301 was downregulated in U2OS and SAOS-2 cells. Importantly, epirubicin significantly increased the levels of miR-1301. Overexpression of miR-1301 suppressed proliferation and promoted apoptosis. Interestingly, those effects were enhanced by epirubicin. In contrast, miR-1301 depletion attenuated the epirubicin-mediated anti-osteosarcoma effect. miR-1301 negatively regulated the expression of TRIAP1 in U2OS and SAOS-2 cells. Furthermore, epirubicin inhibited the mRNA and protein levels of TRIAP1 by upregulating miR-1301 levels. Epirubicin suppressed cell proliferation by downregulating TRIAP1. CONCLUSION: miR-1301 was implicated in the chemosensitivity of osteosarcoma to epirubicin by modulating TRIAP1.

Effect of silencing CXCR4 gene on proliferation, invasion and epithelial-mesenchymal transition related protein expressions in HeLa cells of human cervical cancer / 肿瘤研究与临床

Objective To investigate the effects of small interfering RNA (siRNA) CXCR4 gene on proliferation, invasion and epithelial-mesenchymal transition factors including Twist, Slug and E-cadherin (E-cad)in HeLa cells of human cervical carcinoma.Methods HeLa cells were cultured in vitro and CXCR4-siRNA was transfected into HeLa cells. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the changes of cell proliferation. Transwell experiment was used to observe the changes of cell invasion, and the expressions of CXCR4,Twist,Slug and E-cad protein were detected by Western blot.Results Compared with the blank control group and the negative control group,CXCR4-siRNA transfected into HeLa cells significantly inhibited the expression of CXCR4 protein (0.959±0.197, 0.932±0.141 vs. 0.379±0.022 respectively; F=16.286, P =0.004). MTT results showed that the proliferation of HeLa cells was inhibited at 48 h and 72 h (48 h: 0.846 ± 0.034, 0.823 ± 0.025 vs. 0.744 ± 0.039, F = 7.379, P= 0.024; 72 h: 0.996±0.026, 0.964± 0.059 vs. 0.829±0.051, F = 10.425, P= 0.011). Transwell results showed that the invasiveness of HeLa cells in CXCR4-siRNA group was inhibited at 48 h and 72 h(cell membrane number,48 h:37.4±8.1,33.6±6.2 vs.25.4 ±3.2, F = 4.830, P= 0.029. 72 h: 48.4 ±7.6, 43.0 ±5.4 vs. 33.4 ±6.7, F = 6.579, P= 0.012). After transfection for 48 h, the expression of Twist and Slug protein was decreased and E-cad protein was increased in CXCR4-siRNA group (Twist: 0.93±0.11, 1.00±0.17 vs. 0.53±0.07, F = 10.962, P= 0.010; Slug: 0.515± 0.084,0.470±0.055 vs.0.190±0.028,F =25.579,P=0.001;E-cad:0.53±0.04,0.55±0.24 vs.1.11±0.14,F =12.494, P= 0.007). Conclusions Silencing CXCR4 gene can inhibit the proliferation and reduce the invasiveness of HeLa cells. Inhibiting EMT of HeLa cells can be achieved by down-regulating the expressions of Twist and Slug protein,and up-regulating the expression of E-cad.

Mobile Applications of Exercise in Patients with Chronic Obstructive Pulmonary Disease:A Pilot Study / 中国康复理论与实践

@#Objective To develop a mobile application and to investigate the feasibility to support exercising in patients with chronic ob-structive pulmonary disease (COPD) in home. Methods TheCOPDmobile application include 6 function modules including exercising, exercising reminder, ranking, the basic knowledge of disease, exercising statistics and questionnaire collecting. 15 eligible patients with COPD were recruited in the outpatient departments of a tertiary hospital in Beijing by convenience sampling method from August to Decem-ber, 2015. They received Redmi 2a phones and were taught to use COPD application pre-installed in phones. They were asked to useCOPDapplication to support exercising at home for 1 month. Study outcomes included user satisfactions and exercising data. Results 14 (93.3%) patients thoughtCOPDapplications were helpful for exercising, 12 (80%) patients were satisfied withCOPDapplication. Some patients reported difficulty in manipulating smartphones and mobile applications. Some suggestions were put forward, such as, the stability and speed of data transmission should be improved and the sound of metronome should be increased. They exercised (44.6±8.4) minutes ev-ery day for (4.9±1.1) days per week in the latest 1 month. Conclusion Patients with COPD were highly satisfied withCOPDapplication.

Diagnosis,treatment and prognosis of fetal hydrocephalus / 中华实用儿科临床杂志

Fetal hydrocephalus is a kind of congenital malformation,which is characterized by the enlargement of the ventricular system and the obstruction of cerebrospinal fluid.It is not accompanied by a primary brain atrophy,with or without the increase of head circumference.Early diagnosis and early treatment is very important.Without the timely inter-vention,most of the patients will die during the perinatal and infant period,and even the survivors often suffer from cere-bral palsy and mental retardation.However,the long -term prognosis and clinical course of patients with hydrocephalus is not yet fully understood.There is controversy on the factors which influence the long -term outcome,such as the timing of diagnosis of hydrocephalus,the severity of ventricular dilatation,the basic disease and accompanying anomalies.So,should establish comprehensive protocols to avoid and decrease fetal hydrocephalus,as well as pay attention to the clinicopatho-logical research,prenatal diagnosis,perinatal management and early treatment of fetal hydrocephalus.

Heterologous expression and characterization of Klebsiella oxytoca lysine decarboxylase / 生物工程学报

Cadaverine is a biogenic amine that has the potential to become an important platform chemical for the production of industrial polymers, such as polyamides and polyurethanes. We reported here a lysine decarboxylase from Klebsiella oxytoca. The lysine decarboxylase from Klebsiella oxytoca was cloned to Escherichia coli to get the strain LN18. The specific activity of the crude protein from LN18 reached 30 000 U. The molecular weight was about 80 kDa. The optimum temperature and pH of the crude protein were 55 ℃ and 5.5 respectively. The specific activity could keep over 30% at pH 8.0 compared the one at pH 5.5, much difference from Escherichia coli lysine decarboxylase CadA. Mg²⁺ was positive to the specific activity, whereas Fe²⁺, Zn²⁺ and Ca²⁺ were negative.

Study on blood uric acid,lipid levels and perceived stress in Kazakh hypertensive patients / 重庆医学

Objective To study the relationship between Kazakh hypertensive patients with blood uric acid ,lipid levels and psy-chological factors .Methods 90 Kazak patients with hypertension in our hospital from January to August 2012 were randomly se-lected as the hypertension group and contemporaneous 171 non-hypertension patients as the healthy control group .Blood uric acid (UA) ,triglycerides(TG) ,total cholesterol(TC) ,low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein choles-terol(HDL-C) were detected and the perceived stress scale(PSS) was adopted to assess the mental status .Results The mean val-ues of TG ,TC ,HDL and LDL in males of the hypertension group and the mean values of UA ,TG and LDL in females of the hyper-tension group were significantly higher than those in the normal control group after adjusting age and BMI (P<0 .05);in the hyper-tension group ,UA was positively correlated with TC (r=0 .221 ,P<0 .05) .The level of perceived stress was positively correlated TC and LDL(r=0 .473 ,P<0 .05;r=0 .321 ,P<0 .05) and negatively correlated with TG and HDL (r= -0 .461 ,P<0 .05;r= -0 .412 ,P<0 .05) after adjusting age ,sex and BMI .Conclusion The blood lipid level in Kazakh hypertensive patients has certain correlation with the perceived stress .

Evaluation of the therapies for neonatal posthaemorrhagic hydrocephalus / 临床儿科杂志

Although the percentage of preterm infants developing intraventricular haemorrhage (IVH) has been greatly re-duced in the past three decades, increased survival of extremely preterms has meant that severe IVH with subsequent posthae-morrhagic hydrocephalus is still one of the serious unsolved problems for managements of preterms. Therapeutic interventions for posthaemorrhagic hydrocephalus include serial lumbar punctures, repeated aspiration through a ventricular access device, ventriculoperitoneal shunting, drainage, irrigation and fibrinolytic therapy, diuretic therapy treatment to reduce cerebrospinal fluid production, intraventricular fibrinolytic therapy, etc. This review summarizes the current concepts on the pathophysiology, inter-vention indications, and the effectiveness and safety of different interventions of subsequent posthaemorrhagic hydrocephalus.

Ilaprazole in the treatment of duodenal ulcer:a Meta analysis / 中国医师进修杂志

Objective To systematically review the efficacy of ilaprazole in duodenal ulcer by Meta analysis.Methods Randomized controlled trial (RCT) comparing ilaprazole with other proton pump inhibitors in duodenal ulcer were searohed for Meta analysis.Results Six RCT met the inclusion criteria and 1319 patients were included.Meta analysis showed that the healing rate at 4-week in the dose of ilaprazole 10 mg/d was higher than that in control group [ 89.1％ (591/663 ) vs.86.4％ (426/493) ],but there was no significant difference (P＞ 0.05 ).When including high-quality literature,English literature,Chinese literature,or the literature using omeprazole as control,there was no significant difference either (P ＞ 0.05 ).The adverse rate in the two groups had no significant difference [ 9.7％ (64/663) vs.12.6％ (62/493)] (P ＞0.05 ).The 4-week healing rate between the doses of ilaprazole 5 mg/d and 10 mg/d had no significant difference [84.7％(138/163) vs.84.0％(131/156)](P＞0.05).Conclusions Ilaprazole has high healing rate for duodenal ulcer,with low adverse rate.The effect of the dose ot5 mg/d is comparable to 10 mg/d.

Octreotide for advanced hepatocellular carcinoma：Meta-analysis of randomized controlled trials / 中国医师进修杂志

Objective To evaluate the effect of octreotide on the survival of patients with advanced hepatocellular carcinoma(HCC).Methods Electronic databases searches were conducted.Meta-analysis of all randomized controlled trials (RCTs) comparing octreotide versus placebo or no treatment was performed.Results Five RCTs including 579 patients with HCC wers assessed.Meta-analysis of the five studies showed that octreotide had no significant effect on the 6,12 and 24 months survival rates of the patients(6months:OR=1.69,95%CI 0.89-3.21,P=0.11;12 months:OR=2.41,95%CI 0.83-6.93,P=0.10;24months:OR=0.58,95%CI 0.28-1.17,P=0.13).To perform the sensitivity analysis,the blank study,the trial detecting somatostatin receptor expression,the study with short survival or the two trials with short follow-up intervals were excluded,and the results also showed that the octreotide treatment had no significant effect on the 6 and 12 months survival rates.A sensitivity analysis based on two trials indicated that the 24months survival rate of the octreotide group was even lower than that of the control group(OR=0.51,95% CI0.27-0.98,P=0.04).Conclusions This Meta-analysis demonstrates that octreotide could not improve the survival of patients with advanced HCC.The administration of octreotide should not be recommended for the treatment of advaneed HCC.

Establishment of Central Sterilization Supply Department Green Pass Way:Thinking and Practice / 中华医院感染学杂志

OBJECTIVE Discussing the central sterilization and supply department (CSSD) working model,to change CSSD serving concept and to improve clinical satisfaction degree for CSSD. METHODS Nurse in charge set forth an aim to establish CSSD green pass way in 2007. CSSD applied the meticulous administration,prolonged its serving time,assigned trained workers to deliver emergency articles,and prepared the individual packets to meet clinical special needs. RESULTS Clinical satisfaction degree was increased from 58.0% in 2005 to 95.1%in 2008,and complains were decreased from 15 times in 2005 to zero in 2008 (P

The clinical application of mesiolization of molars by segmental arch technique / 实用口腔医学杂志

The first molar of youth is often extracted for seriously caries or without long term good effect after therapy because the first molar is often misunderstood as deciduous tooth by parents and therefore it is regarded as unnecessary to treat. For such young patients, the corresponding ipsilateral first molar caries while third molars (or germ) existing with crown well-developed,we can exact the first molar and move the second molar by segmental arch technique. In this way, we can quickly shift the second molar to the desired location, increases the efficiency and reduce both dental movement and period of treatment.

The clinical research of different dose of esomeprazole-based triple therapies for Helicobacter pylori eradication / 中国医师进修杂志

Objective To compare the efficacy of different dose esomeprazole-based triplen therapies for Helicobacter pylori (Hp)eradication. Methods Two hundred and forty Hp-infected patients were randomly assigned to undergo high-dose (40 mg) or low-dose (20 mg) esomeprasole combined with clarithromycin (500 mg) and amoxicillin (1g) twice a day for one week. Hp eradication test was performed at 4 weeks after the end of treatment to evaluate the response to therapy. Results One hundred and fourteen patients were followed up in high-dose patients, and 104 were Hp eradication. One hundred and thirteen patients were followed up in low-dose patients, and 101 were Hp eradication. There was no significant difference in eradication rate of intention-to treat analysis and per protocol analysis between high-dose and low-dose patients [86.7%(104/120) vs 84.2% (101/120) and 91.2% (104/114) vs 89.4% (101/113), P>0.05]. There was no significant difference in the rate of adverse effect between high-dose and low-dose patients (8.3%(10/120)vs 6.7%(8/120), P>0.05). Conclusion It demonstrates that low-dose esomeprazole-based triple therapy has a similar Hp eradication rate compared with high-dose esomeprazole-based therapy in China.

Optimization of the Preparation Technology of Baicalein Sustained Release Microspheres by Central Composite Design / 中国药房

OBJECTIVE:To optimize the preparation technology of Baicalein sustained release microspheres.METHODS:The microspheres containing baicalein were prepared using solvent evaporation technique.Multiple linear regression equation and quadratic polynomial equation were fitted respectively with the mass ratio of the matrix materials,sodium dodecyl sulfate(SDS)concentration in water phase and drug content as independent variables,and with the accumulative drug release percentage at 2,12 and 24 h(Y1,Y2,Y3)as dependent variables.RESULTS:The baicalein sustained release microspheres prepared in accordance with the above technology were spherical and even.The optimized technical conditions were as follows:the drug content was 25%;the ratio of the matrix materials was 3.5;SDS concentration was 0.08%;Y1,Y2 and Y3 were 21.05%,55.70% and 87.75%,respectively.All the three equations fitted using quadratic polynomial equations achieved good fitting results.CONCLUSION:Central composite design is convenient to use and it has satisfactory predictability thus deserving popularization.

Preparation Technology of Chinese Traditional Medicine Seborrhea Liniment / 中国药房

OBJECTIVE:To study the preparation technology of Chinese traditional medicine Seborrhea liniment.METHODS:The preparation technology of Seborrhea liniment was optimized by orthogonal experiment design L9(34)using 60% alcohol as solvent with the content of Berberine hydrochloride as index for evaluation and with the extraction method,extraction time,amount of solvent and number of extraction times as factors.RESULTS:The optimum preparation technical conditions were as follows:by impregnating method,the extraction time was 48 h using 1 500 mL alcohol and the extraction frequency was 2 times.CONCLUSION:The preparation technology of Chinese traditional medicine Seborrhea liniment is stable and feasible,and it serves as a theoretical basis for the industrial production.

Fingerprint and Quality Evaluation of Apocynum Venetum Leaves / 中国药房

OBJECTIVE:To establish the HPLC fingerprints of Apocynum Venetum leaves, determine the content of Quercetin in the sample and evaluate the variety and quality of commercial Apocynum Venetum leaves. METHODS: Quercetin was separated on Zorbarx-C18(250 mm?4.6 mm, 5 ?m),and the mobile phase consisted of methanol and 0.4% H3PO4(gradient elution) at a flow rate of 0.9 mL?min-1 with the detective wavelength set at 360 nm. RESULTS: The precision of Quercetin was satisfactory(RSD=0.21%,n=6),and the solution of Apocynum Venetum leaves was reproducible and stable within 10 hours. CONCLUSION: This study serves as a scientific basis for the identification and quality evalution of Apocynum Venetum leaves.

Moderate hypothermia post hypoxia-ischemia improves the cerebral energy metabolism in the immature rats / 中国病理生理杂志

AIM: To study the effect of moderate hypothermia on immature rats with hypoxic-ischimic brain damage (HIBD). METHODS: The rats with HIBD were divided into normothermic recovered group (IN) and moderate-hypothermic recovered group (IH). Sham-operated rat pups were normothermic control group (NC) and moderate-hypothermic control group (HC). 0, 2, 6, 24, 48, 72 h after the end of hypoxic-ischemic (HI) insult, the brain was homogenized for measuring glucose and ATP, brain mitochondria was extracted for SDH activity, complex II activity and the capacity of ATP synthesization. RESULTS: In IN group, the brain glucose was significantly lower at 0 h, and recovered as normal at 2 h. The brain ATP and brain-mitochondrial SDH activities were firstly decreased at 2 h, 6 h and then recovered gradually, it was at it's peak value at 72 h. Brain-mitochondrial complex II activity and the capacity of ATP synthesis were recovered at 2 h, but they decreased again at 6 h and came to normal level at 72 h. In moderate-hypothermic group, all the indexes were significantly higher at all the time point than that in IN groups. CONCLUSION: Moderate hypothermia inhibits the decrease in the mitochondrial SDH activity, mitochondrial complex II activity and the capacity of ATP synthesis, increases the brain ATP concentration, improves the energy metabolism, and then protects the brain tissue. [

STUDIES ON POLYSACCHARIDE FROM SARGASSUM THUN-BERGII FOR ITS ABILITY TO SCAVENGE ACTIVE OXYGEN SPECIES Ⅱ On Effect of Far Ultraviolet Radiation on Antioxidation of Polysaccharides / 中国海洋药物

The effect of far ultraviolet radiation on scavenging active oxygen radiacal of polysaccharides from Sargassum thvnbergii and Sargassum kvrneri was studied. The results showed that the scavenging superoxide an ion radiacal levels of two polysaccharides were obviously decreased after 24h by UVc radiation,but the inhibition of the peroxidization of PU-FA was very prominent.The changes of IR spectra before and after far ultraviolet radiation on the two polysac-charide were also studied by differential scanning calorimetry(DSC)and infrared absorption spectroscopy(IR)