HPLC determination of chemical constituents produced in Radix Polygoni Multiflori after processing / 中国中药杂志

<p><b>OBJECTIVE</b>To analysis the changes of two chemical constituents, namely 2, 3-dihydro-3, 5- dihydroxy-6-methyl-4H-pyran-4-one (DDMP) and 5-hydryoxymethyl-furfural (5-HMF) produced in Radix Polygoni Multiflori after processing, with processing time, and to determine the contents of 5-HMF in samples of Radix Polygoni Multiflori and Radix Polygoni Multiflori preparata.</p><p><b>METHOD</b>An HPLC method was applied with a Zobax SB-C18 (3.9 mm x 150 mm, 5 microm) column by a elution using methanol-water (10: 90) as the mobile phase. The detection was set at UV 280 nm.</p><p><b>RESULT</b>The contents of DDMP were increasing with the processing time until 24 hour, followed by a decrease until 60 hour process. The contents of 5-HMF were increasing gradually throughout the 60 hour steaming process. The contents of 5-HMF in 11 samples of Radix Polygoni Multiflori preparata were from 0.013% to 0.101%, and only one in 4 samples of Radix Polygoni Multiflori containing trace amount of 5-HMF.</p><p><b>CONCLUSION</b>The chemical components in Radix Polygoni Multiflori were changed during the processing procedures. Therefore, the processing of Radix Polygoni Multiflori should be controlled and standardized.</p>

Determination of three chemical components in Fructus aurantii immaturus / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the contents of 3 kinds of components in Fructus aurantii immaturus.</p><p><b>METHOD</b>HPLC analysis was performed to detect the contents of hesperidin, naringin and synephrine. The content of volatile oil was detected determined following the method of Chinese pharmacopoeia.</p><p><b>RESULT</b>The contents of hesperidin, naringin, synephrine and volatile oil in ten samples are from 1.25% to 16.6%, 0% to 13.9%, 0.058 5% to 0.676% and 0.1% to 2.2%, respectively.</p><p><b>CONCLUSION</b>The contentre are significant differences of among chemical components in from different samples of Fructus aurantii immaturus are greats.</p>